Browsing by Author "Gentsch, Jon"

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    Determination of the G and P Types of Previously Nontypeable Rotavirus Strains from the African Rotavirus Network, 1996–2004: Identification of Unusual G Types
    (Journal of Infectious Diseases, 2010) Esona, Mathew Dioh; Steele, Duncan; Nyangao, James; Sebunya, Theresia; Dewar, John; Glass, Roger; Gentsch, Jon
    A total of 215 nontypeable rotavirus samples collected from children <5 years of age by members of the African Rotavirus Network were characterized using reverse-transcription polymerase chain reaction analysis and sequencing. The most predominant strain identified was P[8]G1 (46.9%). Genotypes P[8]G10, P[8]G8, P[6]G8, and P[7]G5 were also detected at frequencies varying from 0.5% to 2.3%. This study suggests that reassortment of unusual G types into a background of globally common genotype P[8] strains may be a major mechanism of generating rotavirus diversity. Nucleotide substitutions at the P[8], P[6], and G1 primer binding sites accounted for the failure to type these strains initially. Hence, these findings highlight the need for regular evaluation of rotavirus genotyping methods.