Browsing by Author "Demelash, B."

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    Characterization of mycobacteria isolated from slaughter cattle in pastoral regions of Uganda
    (BMC microbiology, 2007) Oloya, J.; Kazwala, R.; Lund, A.; Opuda-Asibo, J.; Demelash, B.; Skjerve, E.; Johansen, T. B.; Djønne, B.
    Bovine tuberculosis is a zoonotic problem in pastoral cattle and communities in Uganda. Tuberculin tests in pastoral cattle had shown a high herd but low animal prevalence, with a high proportion of avian reactors. No work had been done to identify the mycobacterial species involved. The objective of the study was to isolate and characterise Mycobacterial species causing tuberculous lesions in slaughtered animals. Lesioned organs compatible with bovine tuberculosis in slaughtered cattle from pastoral areas in Uganda were collected and cultured to isolate mycobacteria. AccuProbe culture identification kits for the Mycobacterium tuberculosis complex, Mavium complex and M. avium were used to identify the isolates. Spoligotyping and Insertion Sequence (IS) 1311 and IS1245 Restriction Fragment Length Polymorphism analysis (RFLP) were used to further characterise the isolates.
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    A cross-sectional study of bovine tuberculosis in the transhumant and agro-pastoral cattle herds in the border areas of Katakwi and Moroto districts, Uganda
    (Tropical animal health and production, 2008) Inangolet, F. O.; Demelash, B.; Oloya, J.; Opuda-Asibo, J.; Skjerve, E.
    A study to determine the prevalence of bovine tuberculosis in the transhumant and agropastoral cattle herds in the border areas of Katakwi and Moroto districts in Uganda was carried out from July 2006 to January 2007 using comparative intradermal tuberculin test containing bovine and avian PPDs. A total of 1470 animals, 612 (41.6%) males and 858 (58.4%) females, 883 (60%) young, 555 (37.8%) adult and 32 (2.2%) old animals were included. The study involved a cross-sectional multistage sampling technique with random selection of individual animals from a herd.