Characterization of mycobacteria isolated from slaughter cattle in pastoral regions of Uganda
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Date
2007
Journal Title
Journal ISSN
Volume Title
Publisher
BMC microbiology
Abstract
Bovine tuberculosis is a zoonotic problem in pastoral cattle and communities in Uganda. Tuberculin tests in pastoral cattle had shown a high herd but low animal prevalence, with a high proportion of avian reactors. No work had been done to identify the mycobacterial species involved. The objective of the study was to isolate and characterise Mycobacterial species causing tuberculous lesions in slaughtered animals. Lesioned organs compatible with bovine tuberculosis in slaughtered cattle from pastoral areas in Uganda were collected and cultured to isolate mycobacteria. AccuProbe culture identification kits for the Mycobacterium tuberculosis complex, Mavium complex and M. avium were used to identify the isolates. Spoligotyping and Insertion Sequence (IS) 1311 and IS1245 Restriction Fragment Length Polymorphism analysis (RFLP) were used to further characterise the isolates.
Description
Keywords
Mycobacteria, Cattle, Pastoral regions, Uganda
Citation
Oloya, J., Kazwala, R., Lund, A., Opuda-Asibo, J., Demelash, B., Skjerve, E., ... & Djønne, B. (2007). Characterisation of mycobacteria isolated from slaughter cattle in pastoral regions of Uganda. BMC microbiology, 7(1), 1-7. doi:10.1186/1471-2180-7-95