Browsing by Author "Bukenya, Edmund"

Now showing 1 - 2 of 2
  • Thumbnail Image
    Item
    Effect of Cleome gynandra leaf extract on the estrous cycle and histology of the ovary and uterus of Wistar albino rats
    (Anatomy Journal of Africa, 2019) Monima, Lemuel Ann; Buhari, Muhammad; Lawal, Sodiq; Echoru, Isaac; Ssempijja, Fred; Owembabazi, Elna; Bukenya, Edmund; Mario Edgar, Fernandez Diaz; Archibong Victor, Bassey; Kokas, Ikwap
    Cleome gynandra is a medicinal plant that is used all over Uganda to hasten childbirth because, it possesses the ability to contract the uterus. It is also used as an abortifacient in the first trimester. In this study, the effects of Cleome gynandra were investigated on the estrous cycle and the histology of the ovary and uterus of adult Wistar rat. Twelve adult female Wistar rats of 130-140g average weight were used. These were divided into three groups of four animals each. Group A received distilled water only, while animals in groups B and C received 250mg/kg body weight and 500mg/kg body weight of extract, orally and daily respectively. Monitoring of estrous cycle continued throughout the three weeks of extract administration. After three weeks, the ovaries and uteri were excised and processed for histological examination. In the ovary, there was a reduction in number of primordia, primary, secondary and graafian follicles in the treated groups. Vacuolations were common to both the ovarian and uterine tissues of treated animals. The estrous cycle of Group B and C, showed a mild disruption when compared to animals in Group A. The results showed that the plant extract studied, exerted negative influences on the estrous cycle and histology of the ovary and uterus of Wistar albino rats, suggesting a disturbance on the reproductive health of the animals. Further studies to determine the mechanism of action of Cleome gynandra on the ovary and uterus and the levels of FSH, LH, estradiol and progesterone is recommended.
  • Thumbnail Image
    Item
    Neuroprotective Potential of Lantana Trifolium Ethanolic Extract against Ethambutol Induced Histological Changes in the Optic Nerve
    (Anatomy Journal of Africa, 2017) Owembabazi, Elna; Ninsiima, Herbert Izo; Mario, Edgar Fernandez Diaz; Echoru, Isaac; Lemuel, Ann Monima; Ahimbisibwe, James; Bukenya, Edmund
    Ethambutol is an anti-tuberculosis drug important in treatment of multidrug resistant tuberculosis which is on a rise due to emergence of Human Immunodeficiency Virus. However, it has been associated with side effects on optic nerve histology leading to severe neuropathy. The purpose of this study was to establish the protective potential of Lantana trifolium ethanolic extract against ethambutol induced histological changes in the optic nerve. Twenty five male adult wistar rats of 110-130g average weight were used. These were divided into five groups each comprising five animals. Group A received distilled water only. Group B was treated with 100 mg/kg/day of ethambutol. Groups C, D, and E were treated with 25, 50, and 100 mg/kg/day of Trifolium Extract respectively, one hour before administering 100 mg/kg/day of ethambutol. After five weeks the optic nerves were excised, processed and stained for histological studies. It was observed that Lantana trifolium ethanolic extract had a dose dependent protective potential against ethambutol induced histological changes in the optic nerve. The histology of the optic nerve showed that the numbers of vacuoles were significantly few in group A (2.2±0.37), D (6.2±1.07), and E (5±0.71) when compared against the positive control group (37.4±1.54). Axons were mildly demyelinated in group D and E compared to group B and C. The protective potential of Latana trifolium is possibly present due to its anti-oxidative and anti-inflammatory activities. Studies to determine the exact phytochemical component and mechanism of action responsible for the neuroprotective potential of Latana trifolium should be conducted.