Morphological Identification of Phytophthora a Causal Organism of Pineapple Heart Rot Disease in Uganda

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dc.contributor.authorAkasairi, Ocwa
dc.contributor.authorBua, Bosco
dc.contributor.authorOculi, Jasper
dc.contributor.authorTusiime, Geoffrey
dc.date.accessioned2022-06-09T08:12:50Z
dc.date.available2022-06-09T08:12:50Z
dc.date.issued2018
dc.description.abstractPineapple (Ananas comosus L. Merr) is an economic horticultural crop in Uganda. However, pineapple production is currently being threatened by the latest outbreak of pineapple heart rot disease in Uganda. Yet, information on pineapple heart rot disease causal pathogen is unknown. Therefore, the objective of this study was to identify the pathogen causing pineapple heart rot disease (PHRD) in Uganda. Samples of pineapple leaves with symptoms of PHRD were collected from four districts of Masaka, Luwero, Mukono and Kayunga. Pathogen isolation was done using corn meal agar (CMA) amended with Pimaricin, Ampicillin, Rifampicin Pentachloronitrobenzene, Hymexazol and Benomyl (PARPHB). Macro- and micro- morphological characteristics of the isolates were assessed using Potato dextrose agar (PDA) and V8 media, respectively. Pathogenicity of the isolates was tested using healthy pineapple plants based on Koch’s postulate. The results showed a significant difference (P<0.05) in the growth rates, hyphae diameter and asexual structure dimensions of the isolates. Isolates were characterized by a dense rosette and stoloniferous mycelial growth pattern in PDA media. Although, sporangia were non-caduceus, terminal, papillate and mostly ovoid, obpyriform and limoniform sporangia (32-56μm) with a mean length/breadth ratio of 1.32:1μm were observed. Chlamydospores were spherical and thick-walled (25-42μm diameter) while Sporangiophores branching was sympodial. Based on the morphological characteristics of isolates, it was concluded that Phytophthora nicotianae is the species causing pineapple heart rot disease in Uganda. This study, therefore, represents the first comprehensive information in Uganda. However, molecular identification of the pathogen is recommended to confirm the genetic identity of the species.en_US
dc.identifier.citationAkasairi, O., Bosco, B., Jasper, O., & Geoffrey, T. (2018). Morphological Identification of Phytophthora A Causal Organism of Pineapple Heart Rot Disease in Uganda. International Journal of Phytopathology, 7(1), 07-18. DOI: 10.33687/phytopath.007.01.2367en_US
dc.identifier.other10.33687/phytopath.007.01.2367
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3836
dc.language.isoenen_US
dc.publisherInternational Journal of Phytopathologyen_US
dc.subjectAnanas comosusen_US
dc.subjectHeart roten_US
dc.subjectMorphological identificationen_US
dc.subjectPhytophthoraen_US
dc.titleMorphological Identification of Phytophthora a Causal Organism of Pineapple Heart Rot Disease in Ugandaen_US
dc.typeArticleen_US
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