UCP-LF and other Assay Methods for Schistosome Circulating Anodic Antigen between 1978 and 2022

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dc.contributor.authorFasogbon, Ilemobayo Victor
dc.contributor.authorAja, Patrick Maduabuchi
dc.contributor.authorOndari, Erick Nyakundi
dc.contributor.authorAdebayo, Ismail
dc.contributor.authorEgesa, Moses
dc.contributor.authorTusubira, Deusdedit
dc.contributor.authorSasikumar, Swamiappan
dc.contributor.authorOnohuean, Hope
dc.date.accessioned2023-06-26T16:39:22Z
dc.date.available2023-06-26T16:39:22Z
dc.date.issued2023
dc.description.abstractDetection of circulating anodic antigen (CAA) is known for its high sensitivity in diagnosing schistosomiasis infection, even in low prevalence settings. The Up-Converting Phosphor-Lateral Flow (UCP-LF) assay developed in 2008 presented greater sensitivity than other assay methods in use for CAA detection. Our study aims to comprehensively review all studies conducted in this area and thus generate informed conclusions on the potential for adopting the UCP-LF assay for diagnosing this important yet neglected tropical disease. Using the Preferred Reporting Items for Systematic Reviews and Meta-analysis (PRISMA) guidelines, we generated search criteria to capture all studies in English journals available in the Scopus and PubMed databases on 20 December 2022. A total of 219 articles were identified, and 84 that met the inclusion criteria were retrieved and eventually included in the study. Twelve different assay methods were identified with a noteworthy transition from enzyme-linked immunosorbent assay (ELISA) to the UCP-LF assay, a laboratory-based assay that may be applicable as a point-of-care (POC) diagnostic test for schistosomiasis. Reducing the time, cost, and dependence on specialized laboratory skills and equipment, especially relating to the trichloroacetic acid extraction step and centrifugation in the UCP-LF CAA assay may go a long way to aid its potential as a POC tool. We also propose the development of a CAA-specific aptamer (short protein/antigen-binding oligonucleotide) as a possible alternative to monoclonal antibodies in the assay. UCP-LF has great potential for POC application.en_US
dc.identifier.citationFasogbon, I. V., Aja, P. M., Ondari, E. N., Adebayo, I., Ibitoye, O. A., Egesa, M., ... & Onohuean, H. (2023). UCP-LF and other assay methods for schistosome circulating anodic antigen between 1978 and 2022. Biology Methods and Protocols, 8(1), bpad006.https://doi.org/10.1093/biomethods/bpad006en_US
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8990
dc.language.isoenen_US
dc.publisherBiology Methods and Protocolsen_US
dc.subjectUCP-LFen_US
dc.subjectReviewen_US
dc.subjectAssay-methodsen_US
dc.subjectCirculating-anodic-antigenen_US
dc.subjectSchistosomiasisen_US
dc.titleUCP-LF and other Assay Methods for Schistosome Circulating Anodic Antigen between 1978 and 2022en_US
dc.typeArticleen_US
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