Discovery of a Unique Novel Clade of Mosquito-Associated Bunyaviruses

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dc.contributor.authorMarklewitz, Marco
dc.contributor.authorZirkel, Florian
dc.contributor.authorRwego, Innocent B.
dc.contributor.authorHeidemann, Hanna
dc.contributor.authorTrippner, Pascal
dc.contributor.authorKurth, Andreas
dc.date.accessioned2022-06-13T19:29:10Z
dc.date.available2022-06-13T19:29:10Z
dc.date.issued2013
dc.description.abstractBunyaviruses are the largest known family of RNA viruses, infecting vertebrates, insects, and plants. Here we isolated three novel bunyaviruses from mosquitoes sampled in Côte d'Ivoire, Ghana, and Uganda. The viruses define a highly diversified monophyletic sister clade to all members of the genus Orthobunyavirus and are virtually equidistant to orthobunyaviruses and tospoviruses. Maximal amino acid identities between homologous putative proteins of the novel group and orthobunyaviruses ranged between 12 and 25%. The type isolates, tentatively named Herbert virus (HEBV), Taï virus (TAIV), and Kibale virus (KIBV), comprised genomes with L, M, and S segments of about 7.4 kb, 2.7 kb, and 1.1 kb, respectively. HEBV, TAIV, and KIBV encode the shortest bunyavirus M segments known and did not seem to encode NSs and NSm proteins but contained an elongated L segment with an ∼500-nucleotide (nt) insertion that shows no identity to other bunyaviruses. The viruses replicated to high titers in insect cells but did not replicate in vertebrate cells. The enveloped virions were 90 to 110 nm in diameter and budded at cellular membranes with morphological features typical of the Golgi complex. Viral RNA recovered from infected cells showed 5′-terminal nontemplated sequences of 9 to 22 nt, suggestive of cap snatching during mRNA synthesis, as described for other bunyaviruses. Northern blotting identified RNA species of full and reduced lengths, suggested upon analogy with other bunyaviruses to constitute antigenomic-sense cRNA and transcript mRNAs, respectively. Functional studies will be necessary to determine if this group of viruses constitutes a novel genus in the bunyavirus family.en_US
dc.identifier.citationMarklewitz, M., Zirkel, F., Rwego, I. B., Heidemann, H., Trippner, P., Kurth, A., ... & Junglen, S. (2013). Discovery of a unique novel clade of mosquito-associated bunyaviruses. Journal of virology, 87(23), 12850-12865.https://doi.org/10.1128/JVI.01862-13en_US
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3939
dc.language.isoenen_US
dc.publisherJournal of virologyen_US
dc.titleDiscovery of a Unique Novel Clade of Mosquito-Associated Bunyavirusesen_US
dc.typeArticleen_US
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