Molecular epidemiology of Babesia species, Theileria parva, and Anaplasma marginale infecting cattle and the tick control malpractices in central and eastern Uganda

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dc.contributor.authorTayebwa, Dickson Stuart
dc.contributor.authorVudriko, Patrick
dc.contributor.authorTuvshintulga, Bumduuren
dc.contributor.authorGuswanto, Azirwan
dc.contributor.authorBudiman Nugraha, Arifin
dc.contributor.authorGantuya, Sambuu
dc.contributor.authorEl-Saber Batiha, Gaber
dc.contributor.authorMusinguzi, Simon Peter
dc.contributor.authorKomugisha, Mariam
dc.contributor.authorBbira, Jonh Son
dc.contributor.authorOkwee-Acai, James
dc.contributor.authorTweyongyere, Robert
dc.contributor.authorWampande, Eddie M.
dc.contributor.authorByaruhanga, Joseph
dc.contributor.authorAdjou Moumouni, Paul Franck
dc.contributor.authorSivakumar, Thillaiampalam
dc.contributor.authorYokoyama, Naoaki
dc.contributor.authorIgarashi, Ikuo
dc.date.accessioned2023-02-09T16:43:07Z
dc.date.available2023-02-09T16:43:07Z
dc.date.issued2018
dc.description.abstractEast Coast fever, babesiosis, and anaplasmosis are the major tick-borne diseases affecting cattle productivity in Uganda. The emergence of acaricide-resistant ticks is suspected to have caused a rise in hemoparasites. This study sought to detect and characterize hemoparasites among farms in acaricide-failure hotspots of central as compared to the acaricide-failure naïve areas in eastern Uganda. Nested PCR assays were performed to determine the prevalences of Babesia bovis, Babesia bigemina, Theileria parva, and Anaplasma marginale in cattle blood samples sourced from randomly selected farms. Randomly selected isolates were sequenced to determine the genetic diversity of the parasites using the following marker genes: B. bovis spherical body protein 4, B. bigemina rhoptry-associated protein 1a, T. parva 104 kDa microneme-rhoptry antigen, and A. marginale major surface protein 5. Furthermore, partially and fully engorged adult ticks were collected for taxonomy, and tick-control practices were assessed using a semi-structured questionnaire. The prevalences of B. bigemina, T. parva, and A. marginale in cattle were 17.2, 65.1, and 22.0%, and 10.0, 26.5, and 3% in the central and eastern region, respectively. Whilst, B. bovis was not detected in the farms involved. The sequences for B. bigemina, T. parva, and A. marginale from the central region showed 99% identity with those from the eastern region. Of the 548 ticks collected, 319, 147, 76, and 6 were Rhipicephalus (Boophilus) decoloratus, Rhipicephalus appendiculatus, Amblyomma variegatum, and Rhipicephalus evertsi evertsi, respectively. The Rhipicephalus ticks were more abundant in the central, whereas A. variegatum ticks were more abundant in the eastern region. Tick control malpractices were found in both central and eastern Uganda, and 42 of the 56 surveyed farms lacked appropriate restraining facilities and so they utilized either ropes or a ‘boma’ (enclosure). In summary, B. bigemina, T. parva, A. marginale and their co-infections were more prevalent in the central than eastern region; even though, tick control malpractices were observed in both regions. Therefore, an urgent tick and TBD control strategy is needed.en_US
dc.identifier.citationTayebwa DS, Vudriko P, Tuvshintulga B, Guswanto A, Nugraha AB, Gantuya S, Batiha GE-Saber, Musinguzi SP, Komugisha M, Bbira JS, Okwee-Acai J, Tweyongyere R, Wampande EM, Byaruhanga J, Adjou Moumouni PF, Sivakumar T, Yokoyama N, Igarashi I, Molecular epidemiology of Babesia species, Theileria parva, and Anaplasma marginale infecting cattle and the tick control malpractices in central and eastern Uganda, Ticks and Tick-borne Diseases (2018), https://doi.org/10.1016/j.ttbdis.2018.06.012en_US
dc.identifier.urihttps://doi.org/10.1016/j.ttbdis.2018.06.012
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7698
dc.language.isoenen_US
dc.publisherTicks and tick-borne diseasesen_US
dc.subjectAcaricide failureen_US
dc.subjectCattleen_US
dc.subjectMolecular epidemiologyen_US
dc.subjectTick-borne infectionsen_US
dc.subjectTick control malpracticesen_US
dc.titleMolecular epidemiology of Babesia species, Theileria parva, and Anaplasma marginale infecting cattle and the tick control malpractices in central and eastern Ugandaen_US
dc.typeArticleen_US
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