Identification of Schistosoma mansoni eggs and other soil transmitted intestinal parasites in stool using Odongo-Aginya method
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Date
2019
Journal Title
Journal ISSN
Volume Title
Publisher
Research Square
Abstract
Objective World Health Organisation recommends Kato-Katz technique for quantitative diagnosis of
Schistosoma mansoni and other soil transmitted intestinal parasites in field research. However, for better
visibility especially in hard stool specimens, the prepared slides in Kato-Katz technique are mostly
examined after 1-2 hours. This longer clearing time over clear the eggs of parasites with thin cell walls in
glycerine and morphology of the eggs are not often preserved in Kato-Katz method. These disadvantages
are overcome using Odongo-Aginya method as illustrated by parasite eggs in the results.
Result
The
Odongo-Aginya method uses compound stains of 7.5% nigrosin in 10% formalin and 0.5% eosin yellow in
10% formalin mixed 1:1 ratio. A drop of 50μl of the compound stain is added on 41.7mg of stool and
stirred. This method illustrated the morphological appearance of S. mansoni and other soil transmitted
intestinal parasites clearly when prepared and six weeks after preparation. The prepared slides
maintained the morphological appearance including the eggs of hookworm after being kept in cool dry
place for a long time. This method is reliable, reproducible, cost effective, easy to learn, quick and safe
because of the base formalin, especially when handling specimens from Human Immunodeficiency virus
infected patients.
Description
Keywords
Odongo-Aginya method, Schistosoma mansoni, Intestinal parasites, Illustration in stool
Citation
Byagamy, JP, Malinga, GM, Angwech, H., Echodu, R., & Odongo-Aginya, EI (2019). Identification of Schistosoma mansoni eggs and other soil transmitted intestinal parasites in stool using Odongo-Aginya method. https://doi.org/10.21203/rs.2.14695/v2