Evaluation of the Dynamiker Cryptococcal Antigen Lateral Flow Assay for the Diagnosis of HIV-Associated Cryptococcosis

Simple item page
dc.contributor.authorKwizera, Richard
dc.contributor.authorOmali, Denis
dc.contributor.authorTade, Kiiza
dc.contributor.authorKasibante, John
dc.contributor.authorRutakingirwa, Morris K.
dc.contributor.authorKagimu, Enock
dc.contributor.authorSsebambulidde, Kenneth
dc.contributor.authorWilliams, Darlisha A.
dc.contributor.authorRhein, Joshua
dc.contributor.authorBoulware, David
dc.contributor.authorMeya, David B.
dc.date.accessioned2022-08-18T19:46:43Z
dc.date.available2022-08-18T19:46:43Z
dc.date.issued2021
dc.description.abstractCryptococcal meningitis is a leading cause of meningitis in sub-Saharan Africa. Given the need for rapid point-of-care testing, we evaluated the diagnostic performance of the Dynamiker cryptococcal antigen (CrAg) lateral flow assay (LFA). We assessed the diagnostic performance of the Dynamiker CrAg LFA compared to the IMMY CrAg LFA as the reference standard. We tested 150 serum, 115 plasma, and 100 cerebrospinal fluid (CSF) samples from HIV patients with symptomatic meningitis and 113 serum samples from patients with suspected asymptomatic cryptococcal antigenemia. Compared to the IMMY CrAg LFA, sensitivity of Dynamiker CrAg LFA was 98% in serum, 100% in plasma, 100% in CSF from symptomatic patients and 96% in serum from asymptomatic patients. Specificity was 66% in serum, 61% in plasma, and 91% in CSF from symptomatic patients, and 86% in serum from asymptomatic patients. The positive predictive value was 85% in serum, 82% in plasma, and 96% in CSF from symptomatic patients, and 69% in serum from asymptomatic patients. The negative predictive value was 94% in serum, 100% in plasma, and 100% in CSF from symptomatic patients, and 99% in serum from asymptomatic patients. The interassay reproducibility was 100% across the four sample types with no observed discordant results when Dynamiker CrAg LFA was tested in duplicate. However, a high number of false positives were observed on serum of symptomatic patients (11%), serum of asymptomatic patients (11%) and plasma of symptomatic patients (14%). The Dynamiker CrAg LFA had excellent sensitivity but poor specificity, particularly when tested on serum and plasma.en_US
dc.identifier.citationKwizera, R., Omali, D., Tadeo, K., Kasibante, J., Rutakingirwa, M. K., Kagimu, E., ... & Meya, D. B. (2021). Evaluation of the Dynamiker cryptococcal antigen lateral flow assay for the diagnosis of HIV-associated cryptococcosis. Journal of clinical microbiology, 59(3), e02421-20.https://doi.org/10.1128/JCM.02421-20en_US
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4303
dc.language.isoenen_US
dc.publisherJournal of clinical microbiologyen_US
dc.subjectcryptococcosis, diagnostic techniques, HIV, point-of-care systems, sensitivity and specificityen_US
dc.titleEvaluation of the Dynamiker Cryptococcal Antigen Lateral Flow Assay for the Diagnosis of HIV-Associated Cryptococcosisen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Evaluation of the Dynamiker Cryptococcal Antigen Lateral Flow Assay for the Diagnosis of HIV-Associated Cryptococcosis.pdf
Size:
207.43 KB
Format:
Adobe Portable Document Format
Description:
Evaluation of the Dynamiker Cryptococcal Antigen Lateral Flow Assay for the Diagnosis of HIV-Associated Cryptococcosis
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Medical and Health Sciences