Molecular diagnostics of groundnut rosette disease agents in Uganda: Implications on epidemiology and management of groundnut rosette disease

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dc.contributor.authorKalule Okello, David
dc.contributor.authorAdrogu Ugen, Michael
dc.contributor.authorTukamuhabwa, Phinehas
dc.contributor.authorOchwo- Ssemakula, Mildred
dc.contributor.authorLapaka Odong, Thomas
dc.contributor.authorAdriko, John
dc.contributor.authorKiconco, Faith
dc.contributor.authorMale, Allan
dc.contributor.authorDeom, Carl Michael
dc.date.accessioned2022-02-01T09:22:58Z
dc.date.available2022-02-01T09:22:58Z
dc.date.issued2017
dc.description.abstractThe objective of this study was to use molecular diagnostic tools to detect the agents of groundnut rosette disease (GRD) to guide in varietal development and disease management. Samples were collected from both GRD infected and healthy plants and sites geo-referenced. RNA extraction, cDNA synthesis, polymerase chain reaction (PCR) amplification, electrophoresis, staining and visualization were performed according to standard procedures. Molecular diagnosis of the samples showed various combinations of the GRD agents, some in isolation and others a combination of two or three agents. This distribution is attributed to dependence on the aphid feeding behaviour and pathogenicity of GRD agents. Chlorotic and green rosette symptoms were observed throughout the sampling sites signifying the presence of satellite RNA (sat-RNA) variants. Some plants showing GRD symptoms tested negative for GRD, whereas some healthy-looking plants tested positive for the GRD complexes pointing to the ineffectiveness of phenotypic screening and the need for a molecular diagnostic tool that detects all three GRD agents both in absence or presence of disease symptoms. The absence of groundnut rosette assistor virus (GRAV) in some symptomatic samples signifies that they are epidemiologically dead end sources since GRV and sat-RNA must be packaged within the GRAV coat protein to be aphid transmissible. Oyado (Cassia obtusifolia) tested positive for all the GRD agents making it a potential alternative host. There is an urgent need for validation of the phenotypic screening with molecular tools in efficient diagnosis of the multi-pathogenic GRD in guiding both plant breeding and pathology work.en_US
dc.identifier.citationOkello, D. K., Ugen, M. A., Tukamuhabwa, P., Ochwo-Ssemakula, M., Odong, T. L., Adriko, J., ... & Deom, C. M. (2017). Molecular diagnostics of groundnut rosette disease agents in Uganda: Implications on epidemiology and management of groundnut rosette disease. Journal of plant breeding and crop science, 9(5), 63-70. DOI: 10.5897/JPBCS2016.0630en_US
dc.identifier.other10.5897/JPBCS2016.0630
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/1726
dc.language.isoenen_US
dc.publisherJournal of plant breeding and crop scienceen_US
dc.subjectArachis hypogaea L.en_US
dc.subjectGroundnut rosette diseaseen_US
dc.subjectMolecular diagnosticsen_US
dc.subjectPolymerase chain reaction (PCR)en_US
dc.titleMolecular diagnostics of groundnut rosette disease agents in Uganda: Implications on epidemiology and management of groundnut rosette diseaseen_US
dc.typeArticleen_US
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