Feasibility of Silicon Quantum Dots as a Biomarker for the Bioimaging of Tear Film

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dc.contributor.authorSarwat, Sidra
dc.contributor.authorStapleton, Fiona Jane
dc.contributor.authorPerry Willcox, Mark Duncan
dc.contributor.authorO’Mara, Peter B.
dc.contributor.authorTilley, Richard David
dc.contributor.authorGooding, J. Justin
dc.contributor.authorRoy, Maitreyee
dc.date.accessioned2022-12-18T12:04:06Z
dc.date.available2022-12-18T12:04:06Z
dc.date.issued2022
dc.description.abstractThis study investigated the fluorescence and biocompatibility of hydrophilic silicon quantum dots (SiQDs) that are doped with scandium (Sc-SiQDs), copper (Cu-SiQDs), and zinc (Zn-SiQDs), indicating their feasibility for the bioimaging of tear film. SiQDs were investigated for fluorescence emission by the in vitro imaging of artificial tears (TheraTears®), using an optical imaging system. A trypan blue exclusion test and MTT assay were used to evaluate the cytotoxicity of SiQDs to cultured human corneal epithelial cells. No difference was observed between the fluorescence emission of Sc-SiQDs and Cu-SiQDs at any concentration. On average, SiQDs showed stable fluorescence, while Sc-SiQDs and Cu-SiQDs showed brighter fluorescence emissions than Zn-SiQDs. Cu-SiQDs and Sc-SiQDs showed a broader safe concentration range than Zn-SiQDs. Cu-SiQDs and Zn-SiQDs tend to aggregate more substantially in TheraTears® than Sc-SiQDs. This study elucidates the feasibility of hydrophilic Sc-SiQDs in studying the tear film’s aqueous layer.en_US
dc.identifier.citationSarwat, S.; Stapleton, F.J.; Willcox, M.D.P.; O’Mara, P.B.; Tilley, R.D.; Gooding, J.J.; Roy, M. Feasibility of Silicon Quantum Dots as a Biomarker for the Bioimaging of Tear Film. Nanomaterials 2022, 12, 1965. https://doi.org/10.3390/ nano12121965en_US
dc.identifier.urihttps://doi.org/10.3390/ nano12121965
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/6396
dc.language.isoenen_US
dc.publisherNanomaterialsen_US
dc.subjectDry eye diseaseen_US
dc.subjectTear filmen_US
dc.subjectQuantum dotsen_US
dc.subjectFluorescence imagingen_US
dc.subjectArtificial tearsen_US
dc.titleFeasibility of Silicon Quantum Dots as a Biomarker for the Bioimaging of Tear Filmen_US
dc.typeArticleen_US
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