Genetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritization

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dc.contributor.authorBadji, A.
dc.contributor.authorKwemoi, D. B.
dc.contributor.authorMachida, L.
dc.contributor.authorOkii, D.
dc.contributor.authorMwila, N.
dc.contributor.authorAgbahoungba, S.
dc.contributor.authorKumi, F.
dc.contributor.authorIbanda, A.
dc.contributor.authorBararyenya, A.
dc.contributor.authorSolemanegy, M.
dc.contributor.authorOdong, T.
dc.contributor.authorWasswa, P.
dc.contributor.authorOtim, M.
dc.contributor.authorAsea, G.
dc.contributor.authorOchwo-Ssemakula, M.
dc.contributor.authorTalwana, H.
dc.contributor.authorKyamanywa, S.
dc.contributor.authorRubaihayo, P.
dc.date.accessioned2022-09-06T18:23:56Z
dc.date.available2022-09-06T18:23:56Z
dc.date.issued2020
dc.description.abstractSeveral species of herbivores feed on maize in field and storage setups, making the development of multiple insect resistance a critical breeding target. In this study, an association mapping panel of 341 tropical maize lines was evaluated in three field environments for resistance to fall armyworm (FAW), whilst bulked grains were subjected to a maize weevil (MW) bioassay and genotyped with Diversity Array Technology’s single nucleotide polymorphisms (SNPs) markers. A multi-locus genome-wide association study (GWAS) revealed 62 quantitative trait nucleotides (QTNs) associated with FAW and MW resistance traits on all 10 maize chromosomes, of which, 47 and 31 were discovered at stringent Bonferroni genome-wide significance levels of 0.05 and 0.01, respectively, and located within or close to multiple insect resistance genomic regions (MIRGRs) concerning FAW, SB, and MW. Sixteen QTNs influenced multiple traits, of which, six were associated with resistance to both FAWandMW, suggesting a pleiotropic genetic control. Functional prioritization of candidate genes (CGs) located within 10–30 kb of the QTNs revealed 64 putative GWAS-based CGs (GbCGs) showing evidence of involvement in plant defense mechanisms. Only one GbCG was associated with each of the five of the six combined resistance QTNs, thus reinforcing the pleiotropy hypothesis. In addition, through in silico co-functional network inferences, an additional 107 network-based CGs (NbCGs), biologically connected to the 64 GbCGs, and di erentially expressed under biotic or abiotic stress, were revealed within MIRGRs. The provided multiple insect resistance physical map should contribute to the development of combined insect resistance in maize.en_US
dc.identifier.citationBadji, A., Kwemoi, D. B., Machida, L., Okii, D., Mwila, N., Agbahoungba, S., ... & Rubaihayo, P. (2020). Genetic basis of maize resistance to multiple insect pests: integrated genome-wide comparative mapping and candidate gene prioritization. Genes, 11(6), 689. doi:10.3390/genes11060689en_US
dc.identifier.other10.3390/genes11060689
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4591
dc.language.isoenen_US
dc.publisherGenesen_US
dc.subjectCombined insect resistanceen_US
dc.subjectQTNsen_US
dc.subjectFunctional prioritizationen_US
dc.subjectFall armywormen_US
dc.subjectMaize weevilen_US
dc.subjectStem borersen_US
dc.titleGenetic Basis of Maize Resistance to Multiple Insect Pests: Integrated Genome-Wide Comparative Mapping and Candidate Gene Prioritizationen_US
dc.typeArticleen_US
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