RNAi-derived field resistance to Cassava brown streak disease persists across the vegetative cropping cycle

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dc.contributor.authorOdipio, John
dc.contributor.authorOgwok, Emmanuel
dc.contributor.authorTaylor, Nigel J.
dc.contributor.authorHalsey, Mark
dc.contributor.authorBua, Anton
dc.contributor.authorFauquet, Claude M.
dc.contributor.authorAlicai, Titus
dc.date.accessioned2022-02-16T05:12:16Z
dc.date.available2022-02-16T05:12:16Z
dc.date.issued2014
dc.description.abstractA confined field trial was established to determine durability of RNAi-mediated resistance to Cassava brown streak disease (CBSD). Stem cuttings were obtained from field-grown cassava plants of cv 60444 transgenic for construct p718, consisting of an 894 bp inverted repeat sequence from the Ugandan Cassava brown streak virus (UCBSV) coat protein. Plants were established from three transgenic lines previously shown to provide complete resistance to UCBSV and differing levels of protection to the non-homologous virus species Cassava brown streak virus (CBSV), and grown for 11 months. CBSD symptoms were observed on shoots and storage roots of all non-transgenic cv 60444 control plants and transgenic lines p718–002 and p718–005, but not on p718–001. RT-PCR diagnostic showed tissues of plant lines p718–002 and p718–005 to be infected with CBSV, but free of UCBSV. All leaves and roots of p718–001 plants were confirmed to carry no detectable levels of either pathogen. Plants of cv 60444 in this field trial showed severe cassava mosaic disease symptoms, indicating that presence of replicating geminiviruses did not cause significant suppression of RNAi-mediated resistance to CBSD. Resistance to CBSD across a vegetative cropping cycle confirms earlier field data, and provides an important step in proof of concept for application of RNAi technology to control of CBSD under conditions encountered in farmers’ fields.en_US
dc.identifier.citationJohn Odipio, Emmanuel Ogwok, Nigel J Taylor, Mark Halsey, Anton Bua, Claude M Fauquet & Titus Alicai (2014) RNAi-derived field resistance to Cassava brown streak disease persists across the vegetative cropping cycle, GM Crops & Food, 5:1, 16-19, DOI: 10.4161/ gmcr.26408en_US
dc.identifier.issn2164-5698 (Print) 2164-5701 (Online)
dc.identifier.other10.4161/ gmcr.26408
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/2169
dc.language.isoenen_US
dc.publisherGM Crops & Fooden_US
dc.subjectCassava brown streak diseaseen_US
dc.subjectRNAi-mediated resistanceen_US
dc.subjectCassavaen_US
dc.subjectTransgenicen_US
dc.subjectConfined field trialen_US
dc.subjectUgandaen_US
dc.titleRNAi-derived field resistance to Cassava brown streak disease persists across the vegetative cropping cycleen_US
dc.typeArticleen_US
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