Hippeastrum hybridum anthocyanins as indicators of endpoint in acid – base titrations

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dc.contributor.authorByamukama, Robert
dc.contributor.authorWaninda, Eric Nicholas
dc.contributor.authorMukama, David
dc.contributor.authorNamukobe, Jane
dc.date.accessioned2021-12-13T13:45:27Z
dc.date.available2021-12-13T13:45:27Z
dc.date.issued2016
dc.description.abstractAnthocyanins from Hippeastrum hybridum (Amaryllis) were investigated as indicators of endpoint in acid- base titrations. Extraction of the anthocyanins was done using distilled water, methanol and methanol containing 0.5% acetic acid. The extracts were used in determination of endpoint in titrations between strong acid/strong base, strong acid / weak base, weak acid / strong base and weak acid / weak base at concentration levels of 0.1 M, 0.5 M and 1.0 M. The titres were compared to those obtained using phenolphthalein, methyl orange and mixed indicator. The shelf life of anthocyanin extracts kept in amber bottles at room temperature (16.8 0C – 29.1 0C) was also investigated. The plant extracts gave accurate and precise results in titration of strong acids with strong bases and weak acids against strong bases at all concentrations investigated (0.1, 0.5 and 1.0 M) that were very comparable to the values obtained using phenolphthalein, methyl orange and mixed indicator. In titration of weak acids against weak bases, the plant extracts gave accurate results when concentration was 0.1 M but failed for 0.5 M and 1.0 M. On average the anthocyanins from Hippeastrumhybridum could be used in the titration of strong acid versus strong base, weak acid versus strong base andweak acid versus weak base. The anthocyanins from Hippeastrum hybridum had a good shelf life of 90 dayswhen kept at room temperature. The extracts can thus be used in any place including those that have no access to refrigeration facilities since they can be kept at room temperature for some time before they undergo degradation.en_US
dc.identifier.citationByamukama, R., Waninda, E. N., Mukama, D., & Namukobe, J. (2016). Hippeastrum hybridum anthocyanins as indicators of endpoint in acid–base titrations. International Journal of Biological and Chemical Sciences, 10(6), 2716-2727.DOI: http://dx.doi.org/10.4314/ijbcs.v10i6.25en_US
dc.identifier.issn1997-342X (Online),1991-8631 (Print)
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/443
dc.language.isoenen_US
dc.publisherInternational Journal of Biological and Chemical Sciencesen_US
dc.subjectHippeastrum hybridumen_US
dc.subjectAnthocyaninsen_US
dc.subjectPH indicatorsen_US
dc.subjectAcid-base titrationsen_US
dc.subjectShelf lifeen_US
dc.titleHippeastrum hybridum anthocyanins as indicators of endpoint in acid – base titrationsen_US
dc.typeArticleen_US
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