The effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryos

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dc.contributor.authorBusinge, Edward
dc.contributor.authorBygdell, Joakim
dc.contributor.authorWingsle, Gunnar
dc.contributor.authorMoritz, Thomas
dc.contributor.authorEgertsdotter, Ulrika
dc.date.accessioned2023-02-05T14:50:56Z
dc.date.available2023-02-05T14:50:56Z
dc.date.issued2013
dc.description.abstractSomatic embryogenesis (SE) represents a useful experimental system for studying the regulatory mechanisms of embryo development. In this study, the effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce (Picea abies (L.) Karst) somatic embryos were investigated. Using time lapse photography, we monitored development from proliferation of proembryogenic masses (PEMs) to maturation of somatic embryos in two P. abies cell lines cultured on two maturation treatments. A combination of sugar assays, metabolic and proteomic analyses were used to quantify storage reserves in the mature somatic embryos. The maturation treatment containing a non-permeating osmoticum, polyethylene glycol (PEG, 7.5%) and maltose (3%) as the carbohydrate gave significantly high maturation and low germination frequencies of somatic embryos compared to the treatment with only 3% sucrose. Somatic embryos treated with 3% sucrose contained high levels of sucrose, raffinose and late embryogenesis abundant (LEA) proteins. These compounds are known to be involved in the acquisition of desiccation tolerance during seed development and maturation. In addition the sucrose treatment significantly increased the content of starch in the somatic embryos while the maltose and PEG treatment resulted in somatic embryos with a high content of storage proteins. The high levels of sucrose, raffinose and LEA proteins in the embryos treated with 3% sucrose suggest that sucrose may improve the germination of somatic embryos by promoting the acquisition of desiccation tolerance.en_US
dc.identifier.citationBusinge, E., Bygdell, J., Wingsle, G., Moritz, T., & Egertsdotter, U. (2013). The effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryos. Physiologia plantarum, 149(2), 273-285. doi: 10.1111/ppl.12039en_US
dc.identifier.other10.1111/ppl.12039
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7565
dc.language.isoenen_US
dc.publisherPhysiologia plantarumen_US
dc.subjectCarbohydratesen_US
dc.subjectOsmoticumen_US
dc.subjectStorage reserve accumulationen_US
dc.subjectGerminationen_US
dc.subjectNorway spruce somatic embryosen_US
dc.titleThe effect of carbohydrates and osmoticum on storage reserve accumulation and germination of Norway spruce somatic embryosen_US
dc.typeArticleen_US
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