Exchanging and managing in-vitro elite germplasm to combat Cassava Brown Streak Disease (CBSD) and Cassava Mosaic Disease (CMD) in Eastern and Southern Africa

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dc.contributor.authorTumwegamire, Silver
dc.contributor.authorKanju, Edward
dc.contributor.authorLegg, James
dc.contributor.authorShirima, Rudolph
dc.contributor.authorKombo, Salehe
dc.contributor.authorMkamilo, Geoffrey
dc.contributor.authorMtunda, Kiddo
dc.contributor.authorSichalwe, Karoline
dc.contributor.authorKulembeka, Heneriko
dc.contributor.authorNdyetabura, Innocent
dc.contributor.authorSaleh, Haji
dc.contributor.authorKawuki, Robert
dc.contributor.authorAlicai, Titus
dc.contributor.authorAdiga, Gerald
dc.contributor.authorBenesi, Ibrahim
dc.contributor.authorMhone, Albert
dc.contributor.authorZacarias, Anabela
dc.contributor.authorFenias Matsimbe, Sofrimento
dc.contributor.authorMunga, Theresia
dc.contributor.authorAteka, Elijah
dc.contributor.authorNavangi, Lynet
dc.contributor.authorNarasegowda Maruthi, Midatharahally
dc.contributor.authorMwatuni, Francis
dc.contributor.authorNgundo, George
dc.contributor.authorMwangangi, Maureen
dc.contributor.authorMbugua, Edward
dc.contributor.authorNdunguru, Joseph
dc.contributor.authorRajabu, Cyprian
dc.contributor.authorMark, Deogratius
dc.date.accessioned2022-06-23T18:07:44Z
dc.date.available2022-06-23T18:07:44Z
dc.date.issued2018
dc.description.abstractCassava varieties resistant to cassava mosaic disease (CMD) and cassava brown streak disease (CBSD) are needed for the food and income security of the rural poor in eastern and southern Africa (ESA). The International Institute of Tropical Agriculture led five national cassava breeding programs (Malawi, Mozambique, Kenya, Tanzania and Uganda) in virus-cleaning and exchanging elite cassava germplasm resistant to both diseases. This paper documents the experiences and lessons learned from the process. Thirty-one clones (25 elite, two standard and four national) were submitted by the five breeding programs to the Natural Resources Institute and Kenya Plant Health Inspectorate Services for virus cleaning and indexing. Subsequently, ca 75 invitro virus-indexed plantlets per clone were sent to Genetic Technologies International Limited (GTIL), a private tissue culture (TC) lab in Kenya, and micro-propagated to produce ≥1500 plantlets. After fulfilling all the formal procedures of germplasm exchange between countries ≥300 plantlets per clone were sent to each partner country. National check clones susceptible to CMD/CBSD were sent only to their countries of origin. In each country, the in-vitro plantlets were acclimatized under screen house conditions and transferred to clean isolated sites for field multiplication. All the clones were cleaned of the viruses, except Tomo. The cleaning process was slow for F19-NL, NASE1, and Kibandameno and TC micro-propagation at GTIL was less efficient for Pwani, Tajirika, NASE1, and Okhumelela than for the other clones. Difficulties in cleaning recalcitrant clones affected the timeline for establishing the multi-site evaluation trials in target countries. The initiative is the one of the kind to successfully clean and exchange elite germplasm as a joint action to combat CBSD in ESA. Adequate preparation in terms of infrastructure and personnel are critical to successfully receiving and adapting the indexed in-vitro plants as new germplasm.en_US
dc.identifier.citationTumwegamire, S., Kanju, E., Legg, J., Shirima, R., Kombo, S., Mkamilo, G., ... & Mark, D. (2018). Exchanging and managing in-vitro elite germplasm to combat Cassava Brown Streak Disease (CBSD) and Cassava Mosaic Disease (CMD) in Eastern and Southern Africa. Food Security, 10(2), 351-368. https://doi.org/10.1007/s12571-018-0779-2en_US
dc.identifier.urihttps://doi.org/10.1007/s12571-018-0779-2
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4063
dc.language.isoenen_US
dc.publisherFood Securityen_US
dc.subjectExchangeen_US
dc.subjectIn-vitroen_US
dc.subjectGermplasmen_US
dc.subjectCBSDen_US
dc.subjectCMDen_US
dc.titleExchanging and managing in-vitro elite germplasm to combat Cassava Brown Streak Disease (CBSD) and Cassava Mosaic Disease (CMD) in Eastern and Southern Africaen_US
dc.typeArticleen_US
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