A field-adapted sampling and HPLC quantification method for lumefantrine and its desbutyl metabolite in whole blood spotted on filter paper
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Date
2008
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Chromatography
Abstract
A quantitative reverse-phase HPLC method with UV detection, for lumefantrine (LF) and desbutyllumefantrine
(DLF) in whole blood spotted on filter paperwas developed. The analyteswere stabilized on filter
paper by treatment of blood with phosphoric acid (1.6 mol/L). Halofantrine was used as internal standard
and the analytes were extracted from filter paper using methanol. The methanolic extract was extracted
with di-isopropylether after addition of acidic phosphate buffer (pH 2). Chromatographic separation was
carried out on a Zorbax Eclipse XDB-phenyl column (4.6mm×150mm, particle size 5 m) at a flow rate
of 1 mL/min using a mobile phase of acetonitrile–ammonium acetate buffer (0.1M ammonium acetate
and 0.01M acetic acid, pH 6.5) (10:90). The absorbance of the compounds was monitored at 335 nm. The
average extraction recovery from filter paper ranged between 45–51% for LF and 25–33% for DLF for a
concentration range between 300 and 3000 nM. Inter- and intra-assay coefficients of variation for LF and
DLF were ≤9.2. Limits of quantification for LF and DLF were 300 nM. The method has been applied in
malaria patients. In conclusion, a simple procedure for blood sampling and quantitative measurement
of lumefantrine and desbutyllumefantrine suitable for field studies in resource-limited laboratories was
developed.
Description
Keywords
Lumefantrine, Desbutyllumefantrine, Filter paper
Citation
Ntale, M., Ogwal-Okeng, J. W., Mahindi, M., Gustafsson, L. L., & Beck, O. (2008). A field-adapted sampling and HPLC quantification method for lumefantrine and its desbutyl metabolite in whole blood spotted on filter paper. Journal of Chromatography B, 876(2), 261-265.doi:10.1016/j.jchromb.2008.11.002