Unique Circulating microRNA Profiles in Epidemic Kaposi’s Sarcoma
Loading...
Date
2020
Authors
Muwonge, Haruna
Kasujja, Hassan
Atugonza, Carolyne
Kasolo, Josephine
Lugaajju, Allan
Nfambi, Joshua
Damani, Ali Moses
Sembajwe, Larry Fred
Kimuli, Ivan
Nakazzi, Faith
Journal Title
Journal ISSN
Volume Title
Publisher
Research Square
Abstract
The Human herpesvirus 8 (HHV-8), causes Kaposi's sarcoma (KS). Kaposi sarcoma in HIV/AIDS patients is referred to as epidemic KS, and is the most common HIV-related malignancy worldwide. Lack of a diagnostic assay to detect latent and early stage disease has increased disease morbidity and mortality. Serum miRNAs have previously been used as potential biomarkers of normal physiology and disease. In the current study, we profiled the unique serum miRNAs in patients with epidemic KS to generate baseline data to aid in developing a miRNA-based non-invasive biomarker assay for Epidemic KS.
This was a comparative cross-sectional study involving 27 patients with epidemic KS, and 27 HIVpositive adults with no prior diagnosis, or clinical manifestation of KS. DNA and RNA were isolated from blood and serum collected from study participants respectively. Nested PCR for circulating HHV-8 DNA was performed on the isolated DNA, whereas miRNA library preparation and sequencing for circulating miRNA was performed on the RNA samples. The miRge2 pipeline and EdgeR were used to analyze the
sequencing data.
Description
Keywords
MicroRNA, Circulating microRNA, Kaposi’s Sarcoma, Biomarker, Blood
Citation
Muwonge, H., Kasujja, H., Atugonza, C., Kasolo, J., Lugaajju, A., Nfambi, J., ... & Bwanga, F. (2020). Unique Circulating microRNA Profiles in Epidemic Kaposi’s Sarcoma. https://doi.org/10.21203/rs.3.rs-116856/v1