Comparison of MGIT and Myco/F Lytic Liquid-Based Blood Culture Systems for Recovery of Mycobacterium tuberculosis from Pleural Fluid
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Date
2015
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of clinical microbiology
Abstract
Tuberculosis (TB) is the most frequent cause of exudative
pleural effusions in areas of high TB incidence. Studies have
shown that Mycobacterium tuberculosis is the causative agent in
up to 44% of HIV-seronegative people hospitalized with a pleural
effusion (1–3), and the percentage is higher in HIV-seropositive
people (4). Pleural TB is a paucibacillary disease. The pathogenesis
of a tuberculous pleural effusion is likely due to a delayed hypersensitivity
reaction to M. tuberculosis proteins (for a review, see
reference 5) and not to a large burden of organisms. The scarcity of
organisms makes it difficult to isolate M. tuberculosis from pleural
fluid samples, leading to low rates of culture confirmation.
Rich culture media are generally more sensitive in detecting
M. tuberculosis in sputum and other clinical samples (6). Few
studies have compared different liquid media and examined their
potential role in combination with solid media for the diagnosis of
tuberculous pleurisy. In this study, we compared the Bactec 9120
Myco/F lytic blood culture system (Myco/F lytic) to the Bactec
mycobacterial growth indicator tube (MGIT) 960 system (Becton
Dickinson, Sparks, MD) (with each liquid system used in conjunction
with locally prepared Middlebrook 7H11 solid medium)
with respect to time to positivity (TTP), sensitivity, specificity, and
percent culture yield of M. tuberculosis isolates from pleural fluid.
Description
Keywords
MGIT, Myco/F Lytic Liquid-Based Blood Culture Systems, Mycobacterium tuberculosis, Pleural Fluid
Citation
Harausz, E., Lusiba, J. K., Nsereko, M., Johnson, J. L., Toossi, Z., Ogwang, S., ... & Joloba, M. L. (2015). Comparison of MGIT and Myco/F lytic liquid-based blood culture systems for recovery of Mycobacterium tuberculosis from pleural fluid. Journal of clinical microbiology, 53(4), 1391-1394. doi:10.1128/JCM.02742-14