Development of a Sensitive and Accurate Stable Isotope Dilution Assay for the Simultaneous Determination of Free 4-Hydroxy-2-(E)-Nonenal and 4-Hydroxy-2-(E)-Hexenal in Various Food Matrices by Gas Chromatography–Mass Spectrometry
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Date
2014
Journal Title
Journal ISSN
Volume Title
Publisher
Food analytical methods
Abstract
An analytical method suitable for the determination
of 4-hydroxy-2-(E)-nonenal (HNE) and 4-hydroxy-2-(E)-
hexenal (HHE) in various food matrices was developed and
validated. The method involves the use of deuterated HNE
and HHE as internal standards, extraction of the analytes from
the matrices followed by derivatization and detection with gas
chromatography–mass spectrometry. Four different food ma-
trices were chosen as model systems including vegetable oils,
unprocessed meat, fried potato crisps, and infant formula and
three different extraction techniques suitable for the different
matrices were applied including the Quick Easy Cheap Effec-
tive Rugged Safe method. The simplicity of the extraction
techniques allows the method to be applied for routine anal-
ysis of a large amount of samples. The results verify the
accuracy and reproducibility of the analytical technique and
its ability to provide reliable quantification of both analytes at
concentrations as low as 12.8 ng g−1 in meat samples. Fur-
thermore, a short overview of the levels of HNE and HHE in
several products available in the Belgian market is presented.
Description
Keywords
Lipid oxidation, 4-Hydroxy-2-(E)-nonenal, Hydroxy-2-(E)-hexenal, QuEchERS
Citation
Papastergiadis, A., Mubiru, E., Van Langenhove, H., & De Meulenaer, B. (2014). Development of a sensitive and accurate stable isotope dilution assay for the simultaneous determination of free 4-hydroxy-2-(E)-nonenal and 4-hydroxy-2-(E)-hexenal in various food matrices by gas chromatography–mass spectrometry. Food analytical methods, 7, 836-843. DOI 10.1007/s12161-013-9689-2