Preliminary evaluation of a Trypanosoma brucei FG-GAP repeat containing protein of mitochondrial localization [version 1; peer review: 1 approved, 1 approved with reservations]

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dc.contributor.authorNamyanja, Monica
dc.contributor.authorXu, Zhi-Shen
dc.contributor.authorMugasa, Claire Mack
dc.contributor.authorLun, Zhao-Rong
dc.contributor.authorMatovu, Enock
dc.contributor.authorChen, Zhengjun
dc.contributor.authorLubega, George W.
dc.date.accessioned2022-11-23T12:16:19Z
dc.date.available2022-11-23T12:16:19Z
dc.date.issued2019
dc.description.abstractTrypanosoma brucei, a causative agent of African Trypanosomiasis, is known to cross the blood brain barrier during the second stage of the disease. It was previously suggested that this parasite crosses the blood brain barrier in a manner similar to that of lymphocytes. This would imply that trypanosomes possess integrins that are required to interact with adhesion molecules located on the blood brain barrier microvascular endothelial cells, as a first step in traversal. To date, no T. brucei integrin has been described. However, one T. brucei putative FG-GAP repeat containing protein (typical of integrins) encoded by the Tb927.11.720 gene, was predicted to be involved in cell-cell/cell-matrix adhesion. Therefore, this study sought to characterize a putative FG-GAP repeat containing protein (FG-GAP RCP) and to determine its cellular localization as a basis for further exploration of its potential role in cell-cell or cell-matrix adhesion. Methods: In this study, we successfully cloned, characterized, expressed and localized this protein using antibodies we produced against its VCBS domain in T. brucei. Results: Contrary to what we initially suspected, our data showed that this protein is localized to the mitochondria but not the plasma membrane. Our data showed that it contains putative calcium binding motifs within the FG-GAP repeats suggesting it could be involved in calcium signaling/binding in the mitochondrion of T. brucei. Conclusion: Based on its localization we conclude that this protein is unlikely to be a trypanosomal integrin and thus that it may not be involved in traversal of the blood brain barrier. However, it could be involved in calcium signaling in the mitochondrion.en_US
dc.identifier.citationNamyanja, M., Xu, Z. S., Mugasa, C. M., Lun, Z. R., Matovu, E., Chen, Z., & Lubega, G. W. (2019). Preliminary evaluation of a Trypanosoma brucei FG-GAP repeat containing protein of mitochondrial localization. AAS Open Research, 2, 165. https://doi.org/10.12688/aasopenres.12986.1en_US
dc.identifier.urihttps://doi.org/10.12688/aasopenres.12986.1
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/5374
dc.language.isoenen_US
dc.publisherAAS Open Researchen_US
dc.subjectTrypanosoma bruceien_US
dc.subjectFG-GAP repeaten_US
dc.subjectIntegrinen_US
dc.subjectMitochondrionen_US
dc.subjectCalcium signalingen_US
dc.titlePreliminary evaluation of a Trypanosoma brucei FG-GAP repeat containing protein of mitochondrial localization [version 1; peer review: 1 approved, 1 approved with reservations]en_US
dc.typeArticleen_US
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