In vitro somatic embryogenesis and regeneration potential of two potato varieties in Uganda

Simple item page
dc.contributor.authorByarugaba, Arinaitwe Abel
dc.contributor.authorEric, Magembe
dc.contributor.authorAlex, Barekye
dc.contributor.authorSkilton, Rob
dc.date.accessioned2022-02-22T18:32:37Z
dc.date.available2022-02-22T18:32:37Z
dc.date.issued2018
dc.description.abstractIn vitro somatic embryogenesis and regeneration are important techniques for crop improvement and mass propagation. This study was conducted to establish and optimize a regeneration system for agronomically important potato cultivars (‘Victoria’ and ‘Rutuku’) of Solanum tuberosum L. in Uganda. Completely randomized design experiments were set up at Bioscience East and Central Africa (BecA) Tissue Culture Laboratory for this purpose. Callus induction and plant regeneration were initiated on internodes and leaf segments of the two potato cultivars in vitro on Murashige and Skoog (MS) medium supplemented with different phytohormones that included auxins such as α naphthalene acetic acid (NAA), cytokinins like benzyl amino purine (BAP), gibberellic acid (GA3), 2,4-dichlorophenoxyacetic acid (2,4-D) and Zeatin at varying concentrations. Callus response depended on the genotype, the concentrations and composition of growth substances. Hormone combination MS + Sucrose 30% + NAA (2 mg/l) + 2,4-D (2 mg/l) + KN (2 mg/l) was found to have the highest callusing rate, that is, 95 and 53%, respectively for internodes and leaf explants. The calli formed from internodes and leaves were friable and soft. The callus colour in all cases ranged from light brown to light cream and in some cases to light green. Shoot bud initiation was observed in all regeneration culture media, with media combination MS + KN (2 mg/l) + Zeatin (2 mg/l) giving the highest shooting rate. The intervening callus phase led to less number of shoot buds for each callus leading to long incubation period. The study showed that it is possible to regenerate potato cultivars (‘Victoria’ and ‘Rutuku’) from cell suspension culture using Murashige and Skoog (MS) medium supplemented with different phytohormones. Combination of MS with NAA + 2,4-D + Kinetin is suitable for callus induction while MS + kinetin and zeatin was better for shoot induction on calli of potato cultivars ‘Victoria’ and ‘Rutuku’.en_US
dc.identifier.citationByarugaba, A. A., Eric, M., Alex, B., & Skilton, R. (2018). In vitro somatic embryogenesis and regeneration potential of two potato varieties in Uganda. African Journal of Biotechnology, 17(15), 515-520. DOI: 10.5897/AJB2018.16431en_US
dc.identifier.issn1684-5315
dc.identifier.other10.5897/AJB2018.16431
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/2260
dc.language.isoenen_US
dc.publisherAfrican Journal of Biotechnologyen_US
dc.subjectRegenerationen_US
dc.subjectSomatic embryogenesisen_US
dc.subjectSolanum tuberosum.en_US
dc.titleIn vitro somatic embryogenesis and regeneration potential of two potato varieties in Ugandaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
In vitro somatic embryogenesis and regeneration.pdf
Size:
544.6 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Agricultural and Veterinary Sciences