Development and validation of quantitative PCR assays for HIV-associated cryptococcal meningitis in sub-Saharan Africa: a diagnostic accuracy study
| dc.contributor.author | Mbangiwa, Tshepiso | |
| dc.contributor.author | Kajanga, Cheusisime | |
| dc.contributor.author | Nabila, Youssouf | |
| dc.contributor.author | Alanio, Alexandre | |
| dc.date.accessioned | 2025-04-03T15:00:02Z | |
| dc.date.available | 2025-04-03T15:00:02Z | |
| dc.date.issued | 2024-03-29 | |
| dc.description.abstract | HIV-associated cryptococcal meningitis is the second leading cause of AIDS-related deaths, with a 10-week mortality rate of 25–30%. Fungal load assessed by colony-forming unit (CFU) counts is used as a prognostic marker and to monitor response to treatment in research studies. PCR-based assessment of fungal load could be quicker and less labour-intensive. We sought to design, optimise, and validate quantitative PCR (qPCR) assays for the detection, identification, and quantification of Cryptococcus infections in patients with cryptococcal meningitis in sub-Saharan Africa. | |
| dc.identifier.citation | Mbangiwa, T., Sturny-Leclère, A., Lechiile, K., Kajanga, C., Boyer-Chammard, T., Hoving, J. C., ... & Hafeez, S. (2024). Development and validation of quantitative PCR assays for HIV-associated cryptococcal meningitis in sub-Saharan Africa: a diagnostic accuracy study. The Lancet Microbe, 5(3), e261-e271. | |
| dc.identifier.other | https://doi.org/10.1016/ S2666-5247(23)00362-2 | |
| dc.identifier.uri | https://nru.uncst.go.ug/handle/123456789/10351 | |
| dc.language.iso | en | |
| dc.publisher | The Lancet Microbe | |
| dc.title | Development and validation of quantitative PCR assays for HIV-associated cryptococcal meningitis in sub-Saharan Africa: a diagnostic accuracy study | |
| dc.type | Article |