Molecular characterization of Acinetobacter baumannii from patients with prolonged hospital stays in three tertiary hospitals of Kano Metropolis, Northwestern Nigeria

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dc.contributor.authorBashir, Alkali
dc.contributor.authorAliero, Adamu A.
dc.contributor.authorIdris, Abdurrazak M.
dc.contributor.authorTakalmawa, Hamisu U.
dc.contributor.authorFaruk, Sarkinfada
dc.contributor.authorAgwu, Ezera
dc.date.accessioned2022-12-20T23:02:27Z
dc.date.available2022-12-20T23:02:27Z
dc.date.issued2019
dc.description.abstractAcinetobacter baumannii is one of the most important opportunistic bacterial pathogens that cause serious health care associated complications in hospitalized patients. This leads to prolong hospital stay which increase cost to both healthcare provider and family of the patients. The study aimed at molecular characterisation of A. baumannii from patients with prolonged hospital stays in three tertiary hospitals of Kano Metropolis, Northwestern Nigeria. A total of 401 samples were collected from orthopedic and post-surgical wound infections, urine, urine catheters and nasal intubation. Acinetobacter spp was isolated using standard microbiological methods. Identification of A. baumannii isolates were done using Phynotypic methods such as culture on Leed Acinetobacter medium, conventional biochemical tests and API 20NE. Suspect Acinetobacter species were further identified using polymerase chain reaction (PCR) and Sanger sequence typing methods. Out of 401 samples collected 138 (34.4%) were positives by yield suspect bacterial isolates 14 (10.1%) of which were suspect A. baumannii. The results of confirmatory sequence typing of isolates showed that 9 (6.5%) of suspect Acinetobacter spp were A. baumannii. The result of susceptibility test showed that A. baumannii isolates were highly resistance to Ampicillin\ salbactam 13 (92.8%) and least resistance to Ciprofloxacin 2 (14.3%) and Amikacin 3 (21.4). The results of this finding showed presence of A. baumannii species resistant to conventional antibiotics and associated with prolonged duration of patients admission in the three studied hospitals. There is need for improved sanitary working condition and proper patients management to reduce the spread of this health care associated infection agent.en_US
dc.identifier.citationAlkali, B., Adamu, A. A., Abdurrazak, M. I., Hamisu, U. T., Sarkinfada, F., & Agwu, E. (2019). Molecular characterization of Acinetobacter baumannii from patients with prolonged hospital stays in three tertiary hospitals of Kano Metropolis, Northwestern Nigeria. African Journal of Microbiology Research, 13(27), 510-517.en_US
dc.identifier.issn1996-0808
dc.identifier.otherDOI: 10.5897/AJMR2019.9189
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/6462
dc.language.isoenen_US
dc.publisherAfrican Journal of Microbiology Researchen_US
dc.subjectMolecularen_US
dc.subjectcharacterizationen_US
dc.subjectAcinetobacter baumanniien_US
dc.titleMolecular characterization of Acinetobacter baumannii from patients with prolonged hospital stays in three tertiary hospitals of Kano Metropolis, Northwestern Nigeriaen_US
dc.typeArticleen_US
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