Metabolomic Profiling of the Effects of Melittin on Cisplatin Resistant and Cisplatin Sensitive Ovarian Cancer Cells Using Mass Spectrometry and Biolog Microarray Technology

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dc.contributor.authorAlonezi, Sanad
dc.contributor.authorTusiimire, Jonans
dc.contributor.authorWallace, Jennifer
dc.contributor.authorDufton, Mark J.
dc.contributor.authorParkinson, John A.
dc.contributor.authorYoung, Louise C.
dc.contributor.authorClements, Carol J.
dc.contributor.authorPark, Jin K.
dc.contributor.authorJeon, Jong W.
dc.contributor.authorFerro, Valerie A.
dc.contributor.authorWatson, David G.
dc.date.accessioned2022-12-24T07:08:21Z
dc.date.available2022-12-24T07:08:21Z
dc.date.issued2016
dc.description.abstractIn the present study, liquid chromatography-mass spectrometry (LC-MS) was employed to characterise the metabolic profiles of two human ovarian cancer cell lines A2780 (cisplatin-sensitive)and A2780CR (cisplatin-resistant) in response to their exposure to melittin, a cytotoxic peptide from bee venom. In addition, the metabolomics data were supported by application of Biolog microarray technology to examine the utilisation of carbon sources by the two cell lines. Data extraction with MZmine 2.14 and database searching were applied to provide metabolite lists. Principal component analysis (PCA) gave clear separation between the cisplatin-sensitive and resistant strains and their respective controls. The cisplatin-resistant cells were slightly more sensitive to melittin than the sensitive cells with IC50 values of 4.5 and 6.8 g/mL respectively, although the latter cell line exhibited the greatest metabolic perturbation upon treatment. The changes induced by melittin in the cisplatin-sensitive cells led mostly to reduced levels of amino acids in the proline/glutamine/arginine pathway, as well as to decreased levels of carnitines, polyamines, adenosine triphosphate (ATP) and nicotinamide adenine dinucleotide (NAD+). The effects on energy metabolism were supported by the data from the Biolog assays. The lipid compositions of the two cell lines were quite different with the A2780 cells having higher levels of several ether lipids than the A2780CR cells. Melittin also had some effect on the lipid composition of the cells. Overall, this study suggests that melittin might have some potential as an adjuvant therapy in cancer treatment.en_US
dc.identifier.citationAlonezi, S., Tusiimire, J., Wallace, J., Dufton, M. J., Parkinson, J. A., Young, L. C., ... & Watson, D. G. (2016). Metabolomic profiling of the effects of melittin on cisplatin resistant and cisplatin sensitive ovarian cancer cells using mass spectrometry and biolog microarray technology. Metabolites, 6(4), 35.en_US
dc.identifier.urihttps://www.mdpi.com/160866
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/6522
dc.language.isoenen_US
dc.publisherMetabolitesen_US
dc.subjectmetabolomicsen_US
dc.subjectMelittinen_US
dc.subjectLC-MSen_US
dc.subjectovarian canceren_US
dc.titleMetabolomic Profiling of the Effects of Melittin on Cisplatin Resistant and Cisplatin Sensitive Ovarian Cancer Cells Using Mass Spectrometry and Biolog Microarray Technologyen_US
dc.typeArticleen_US
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