Genetic Characterisation and Diversity Assessment of Potato Genotypes Using SSR Markers
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Date
2017
Journal Title
Journal ISSN
Volume Title
Publisher
Journal of Agricultural Science
Abstract
Information on diversity of genetic materials is vital for choosing parents in a breeding program. The objective
of the study was to determine the pattern and level of genetic diversity among the selected 20 tetraploid potato
genotypes using 16 SSR markers to identify suitable parents for breeding purposes. The microsatellites showed
considerable variation among genotypes and sixty four alleles were amplified by the 16 primer pairs. The
number of polymorphic alleles per locus ranged from 2 to 8 with an average of 3.9. The highest number of null
alleles was observed was six for genotype Nakpot1. The overall size of the amplified product varied from 48 bp
(marker STI0023) to 309 bp (marker STM5121). PIC values ranged from 0.0948 to 0.7832, with an average of
0.4307 per locus. Heterozygosity values ranged from 0.0997 to 0.805 with an average of 0.466919. Significant
positive linear correlations were observed between PIC values and number of alleles (r = 0.905); and
heterozygosity and number of alleles (r = 0.8659) at p < 0.001. Cluster analysis separated the genotypes into
three different groups. The genetic distance between clones ranged from 1 to 5.7. Cruza had the highest genetic
distance while the shortest genetic distance was observed between 396026.103 and 396034.104. The
microsatellites used in this study provided useful information regarding the variability of the tested genotypes
and their selection for breeding purposes.
Description
Keywords
Genetic variation, Polymorphism, Clustering, Heterozygosity, Microsatellites, Uganda
Citation
Namugga, P., Sibiya, J., Melis, R., & Barekye, A. (2017). Genetic characterisation and diversity assessment of potato genotypes using SSR markers. Journal of Agricultural Science, 9(8). doi:10.5539/jas.v9n8p99