Evaluation of the Xpert MTB/RIF test for the diagnosis of childhood pulmonary tuberculosis in Uganda: a cross-sectional diagnostic study

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dc.contributor.authorSekadde, Moorine Penninah
dc.contributor.authorWobudeya, Eric
dc.contributor.authorJoloba, Moses L.
dc.contributor.authorSsengooba, Willy
dc.contributor.authorKisembo, Harriet
dc.contributor.authorMusoke, Philippa
dc.contributor.authorBakeera-kitaka, Sabrina
dc.date.accessioned2021-12-16T05:55:33Z
dc.date.available2021-12-16T05:55:33Z
dc.date.issued2013
dc.description.abstractBackground: The diagnosis of childhood tuberculosis remains a challenge worldwide. The Xpert MTB/RIF test, a rapid mycobacteria tuberculosis diagnostic tool, was recommended for use in children based on data from adult studies. We evaluated the performance of the Xpert MTB/RIF test for the diagnosis of childhood pulmonary tuberculosis using one induced sputum sample and described clinical characteristics associated with a positive Xpert MTB/RIF test. The sputum culture on both Lowenstein-Jensen (LJ) and Mycobacteria Growth Indicator Tube (MGIT) was the gold standard. Methods: We consecutively enrolled 250 Ugandan children aged 2 months to 12 years with suspected pulmonary tuberculosis between January 2011 and January 2012 into a cross-sectional diagnostic study at a tertiary care facility in Uganda. Results: We excluded data from 15 children (13 contaminated culture and 2 indeterminate MTB/RIF test results) and analysed 235 records. The Xpert MTB/RIF test had a sensitivity of 79.4% (95% CI 63.2 - 89.7) and a specificity of 96.5% (95% CI 93 – 98.3). The Xpert MTB/RIF test identified 13 of the 14 (92.9%) smear positive-culture positive and 14 of the 20 (70%) smear negative -culture positive cases. The Xpert MTB/RIF identified twice as many cases as the smear microscopy (79.4% Vs 41.2%). Age>5 years (OR 3.3, 95% CI 1.4 – 7.4, p value 0.005), a history of Tuberculosis (TB) contact (OR 2.4, 95% CI 1.1 – 5.2, p value 0.03), and a positive tuberculin skin test (OR 4.1, 95% CI 1.7 – 10, p value 0.02) was associated with a positive Xpert MTB/RIF test. The median time to TB detection was 49.5 days (IQR 38.4-61.2) for LJ, and 6 days (IQR 5 – 11.5) for MGIT culture and 2 hours for the Xpert MTB/RIF test.en_US
dc.identifier.citationSekadde, M.P., Wobudeya, E., & Joloba, M., et. al (2013). Evaluation of the Xpert MTB/RIF test for the diagnosis of childhood pulmonary tuberculosis in Uganda: a cross-sectional diagnostic study. BioMed Central: BMC Infectious Diseases, 13:133en_US
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/685
dc.language.isoenen_US
dc.publisherBioMed Centralen_US
dc.subjectChildrenen_US
dc.subjectPulmonary tuberculosisen_US
dc.subjectSensitivityen_US
dc.subjectSpecificityen_US
dc.subjectXpert MTB/RIFen_US
dc.titleEvaluation of the Xpert MTB/RIF test for the diagnosis of childhood pulmonary tuberculosis in Uganda: a cross-sectional diagnostic studyen_US
dc.typeArticleen_US
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