Fine Mapping of Lr49 Using 90K SNP Chip Array and Flow-Sorted Chromosome Sequencing in Wheat

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dc.contributor.authorNsabiyera, Vallence
dc.contributor.authorBaranwal, Deepak
dc.contributor.authorQureshi, Naeela
dc.contributor.authorKay, Pippa
dc.contributor.authorForrest, Kerrie
dc.contributor.authorValárik, Miroslav
dc.contributor.authorDoležel, Jaroslav
dc.contributor.authorHayden, Matthew J.
dc.contributor.authorBariana, Harbans S.
dc.contributor.authorBansal, Urmil K.
dc.date.accessioned2023-01-27T08:42:15Z
dc.date.available2023-01-27T08:42:15Z
dc.date.issued2020
dc.description.abstractLeaf rust, caused by Puccinia triticina, threatens global wheat production due to the constant evolution of virulent pathotypes that defeat commercially deployed all stageresistance (ASR) genes in modern cultivars. Hence, the deployment of combinations of adult plant resistance (APR) and ASR genes in new wheat cultivars is desirable. Adult plant resistance gene Lr49 was previously mapped on the long arm of chromosome 4B of cultivar VL404 and flanked by microsatellite markers barc163 (8.1 cM) and wmc349 (10.1 cM), neither of which was sufficiently closely linked for efficient marker assisted selection. This study used high-density SNP genotyping and flow sorted chromosome sequencing to fine-map the Lr49 locus as a starting point to develop a diagnostic marker for use in breeding and to clone this gene. Marker sunKASP_21 was mapped 0.4 cM proximal to Lr49, whereas a group of markers including sunKASP_24 were placed 0.6 cM distal to this gene. Testing of the linked markers on 75 Australian and 90 European cultivars with diverse genetic backgrounds showed that sunKASP_21 was most strongly associated with Lr49. Our results also show that the Lr49 genomic region contains structural variation relative to the reference stock Chinese Spring, possibly an inverted genomic duplication, which introduces a new set of challenges for the Lr49 cloning.en_US
dc.identifier.citationNsabiyera V, Baranwal D, Qureshi N, Kay P, Forrest K, Valárik M, Doležel J, Hayden MJ, Bariana HS and Bansal UK (2020) Fine Mapping of Lr49 Using 90K SNP Chip Array and Flow-Sorted Chromosome Sequencing in Wheat. Front. Plant Sci. 10:1787. doi: 10.3389/fpls.2019.01787en_US
dc.identifier.other10.3389/fpls.2019.01787
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7315
dc.language.isoenen_US
dc.publisherFrontiers in Plant Scienceen_US
dc.subjectAdult plant resistanceen_US
dc.subjectChromosome sortingen_US
dc.subjectInfinium iSelect 90K SNP arrayen_US
dc.subjectLeaf rusten_US
dc.subjectMarker assisted breedingen_US
dc.titleFine Mapping of Lr49 Using 90K SNP Chip Array and Flow-Sorted Chromosome Sequencing in Wheaten_US
dc.typeArticleen_US
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