Fruit Set and Plant Regeneration in Cassava Following Inter specific Pollination with Castor Bean

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dc.contributor.authorBaguma, J.K.
dc.contributor.authorMukasa, S.B.
dc.contributor.authorKawuki, R.
dc.contributor.authorTugume, A.K.
dc.contributor.authorButtibwa, M.
dc.contributor.authorNalela, P.
dc.contributor.authorEyokia, M.
dc.contributor.authorOshaba, B.
dc.contributor.authorCeballos, H.
dc.contributor.authorLentini, Z.
dc.contributor.authorBaguma, Y.
dc.date.accessioned2022-03-07T12:52:53Z
dc.date.available2022-03-07T12:52:53Z
dc.date.issued2019
dc.description.abstractThe increasing demand for cassava (Manihot esculenta Crantz) for food and non-food uses in the tropics necessitates that its breeding for increased root productivity be made faster. The characteristic long breeding cycle and heterozygous nature of this crop, pose a major obstacle to its rapid genetic improvement. This study aimed at inter-pollinating cassava with castor bean (Ricinus communis), with a purpose of inducing and regenerating cassava doubled haploids (DHs). A total of 3,349 flowers from twelve elite cassava varieties were inter-pollinated with caster bean. A total of 803 fruits were harvested for early embryo rescue and/or ovule culture. Of these, three were dissected to obtain seven unique embryos, while 800 were dissected to obtain 1312 young ovules, all of which were cultured in vitro. Overall, 82 (6.25%) of the cultured ovules formed callus that originated from the embryosac region, which is haploid. Four out of seven rescued embryos (57.1%) regenerated into plantlets. Ploidy analyses of 24 samples using flow cytometry revealed that 23 of the analysed samples were diploid. However, one callus sample was anueploid. Only one sample had an exceptionally high level of homozygosity ( 84.2%). These findings lay a foundation for future research aimed at induction of haploids in cassava.en_US
dc.identifier.citationBaguma, J. K., Mukasa, S. B., Kawuki, R., Tugume, A. K., Buttibwa, M., Nalela, P., ... & Baguma, Y. (2019). Fruit set and plant regeneration in cassava following interspecific pollination with castor bean. African Crop Science Journal, 27(1), 99-118. https://dx.doi.org/10.4314/acsj.v27i1.8en_US
dc.identifier.uriDOI: https://dx.doi.org/10.4314/acsj.v27i1.8
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/2483
dc.language.isoenen_US
dc.publisherAfrican Crop Science Journalen_US
dc.subjectDoubled haploiden_US
dc.subjectEmbryo rescueen_US
dc.subjectOvule cultureen_US
dc.subjectPloidyen_US
dc.titleFruit Set and Plant Regeneration in Cassava Following Inter specific Pollination with Castor Beanen_US
dc.typeArticleen_US
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