Profile of T Cell Recognition of HIV Type 1 Consensus Group M Gag and Nef Peptides in a Clade A1- and D-Infected Ugandan Population

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dc.contributor.authorSerwanga, Jennifer
dc.contributor.authorMugaba, Susan
dc.contributor.authorPimego, Edward
dc.contributor.authorNanteza, Bridget
dc.contributor.authorLyagoba, Fred
dc.contributor.authorNakubulwa, Susan
dc.contributor.authorHeath, Laura
dc.contributor.authorNsubuga, Rebecca N.
dc.contributor.authorNdembi, Nicaise
dc.contributor.authorGotch, Frances
dc.contributor.authorKaleebu, Pontiano
dc.date.accessioned2022-05-17T17:54:22Z
dc.date.available2022-05-17T17:54:22Z
dc.date.issued2012
dc.description.abstractReagents for evaluating non-clade B HIV-specific T cell responses are uncommon. Peptides based on highly conserved HIV-1 consensus group M sequences that are phylogenetically closer to most circulating strains may provide potential alternative reagents in populations with diverse infections, and may be relevant for vaccine design. Recognition of such reagents in clade A1-and D-infected populations has not been previously evaluated. Interferon (IFN)-c ELISpot assay was used to evaluate T cell recognition of Gag and Nef peptides based on consensus group M sequences in 50 treatment-naive adults predominantly infected with HIV-1 clades A1 and D. Gag-induced T cell responses were correlated with gag sequence diversity. Infecting clades were determined from gag sequences for 45 of the 50 subjects as 40% clade A1 (18/45), 45% clade D (20/45), 2% clade C (1/45), 2% A1/C recombinant (1/45), 2% A1/D (1/45), 7% CRF10_CD (3/45), and 2% U (unclassifiable) (1/45). The mean genetic divergence and diversity of clade A and D gag region compared to group M consensus sequences at synonymous and nonsynonymous nucleotide and amino acid levels were not always significant. Gag peptides were targeted at significantly higher frequency [88% (44/50)] than Nef [64% (32/50)]; p = 0.014, although their mean IFN-c magnitudes were comparable ([3703 (95% CI 2567–4839)] vs. [2120 (95% CI 478–3762)]), respectively. Measurable virus-induced IFN-c responses were detected in 96% (48/50) individuals, primarily targeting the more conserved Gag p24 and Nef central core regions. Use of these reagents to screen for HIV-specific IFN-c responses may mitigate the challenge of viral diversity; although this targeting is apparently biased toward a few highly conserved epitopes.en_US
dc.identifier.citationSerwanga, J., Mugaba, S., Pimego, E., Nanteza, B., Lyagoba, F., Nakubulwa, S., ... & Kaleebu, P. (2012). Profile of T cell recognition of HIV type 1 consensus group M Gag and Nef peptides in a clade A1-and D-infected Ugandan population. AIDS research and human retroviruses, 28(4), 384-392. DOI: 10.1089/aid.2011.0175en_US
dc.identifier.other10.1089/aid.2011.0175
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3249
dc.language.isoenen_US
dc.publisherAIDS research and human retrovirusesen_US
dc.subjectT Cellen_US
dc.subjectHIVen_US
dc.subjectNef Peptidesen_US
dc.subjectClade A1-en_US
dc.subjectUgandan Populationen_US
dc.titleProfile of T Cell Recognition of HIV Type 1 Consensus Group M Gag and Nef Peptides in a Clade A1- and D-Infected Ugandan Populationen_US
dc.typeArticleen_US
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