Identification of genes associated with resistance to disinfectants in the most common resistant bacteria strain of Bacteria isolated from Salon Tools in Ishaka Town, Bushenyi District, Uganda

Simple item page
dc.contributor.authorSolange, Gahongayire
dc.contributor.authorNamatovu, Alice
dc.contributor.authorDrago Kato, Charles
dc.date.accessioned2023-01-24T17:06:24Z
dc.date.available2023-01-24T17:06:24Z
dc.date.issued2022
dc.description.abstractThis study identified the genes associated with resistance to disinfectants in the most common resistant bacteria strain of Bacteria isolated from Salon Tools in Ishaka Town, Bushenyi District, Uganda. Twenty-five (25) Salon owners/ Head operators were enrolled in this study to determine the level of awareness about the biosafety practice of salons operation using semi-structured questionnaire. Additionally, total of 125 swab samples were collected from different salons tools (combs, brushes, scissors, clippers and shaving machines) and processed using standard Microbiological methods for isolation of bacteria. The isolated bacteria were identified using standard phenotypic methods including analytical profile index (API). Quaternary ammonium compounds (qac) genes (qacA/B and C) associated with disinfectant resistances were detected from disinfectant resistant Staphylococcus aureus using Multiplex polymerase chain reaction (PCR) and Sanger sequencing methods. Results obtained from this study showed that a significant number of salon operators (p≤0.05) were aware about the important of disinfectant usage, disinfect tool(s) after use and washing hands /tools after use. Among the 125 swabbed samples collected from the salons, 78 (65.5%) were contaminated with different bacterial species. Clippers were found to have higher (25.6%) contamination among the salon tools from which samples were collected. Of all the tested disinfectants, most resistance was shown with Sodium hypochloride1%. Out of the eight (8) bacterial isolates that were analyzed for qac genes, 2(25%) isolates (STP6 and STP9) were found to be qac A/B positive while 2 (25%) isolates (STP8 and STP9) were found to be qac C gene positive. In conclusion, this study showed that, majority of the salon operators was aware about the biosafety practice of salon operation despite the higher contaminations of salons tools. qac A and qac C were detected among some S. aureus isolated. As recommendations, further study should be done and biosafety guideline should be reviewed.en_US
dc.identifier.citationSolange, G., Namatovu, A., & Kato, C. D. Identification of genes associated with resistance to disinfectants in the most common resistant bacteria strain of Bacteria isolated from Salon Tools in Ishaka Town, Bushenyi District, Uganda. IAA Journal of Biological Sciencesen_US
dc.identifier.issn2636-7254
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7169
dc.language.isoenen_US
dc.publisherIAA Journal of Biological Sciencesen_US
dc.subjectIdentificationen_US
dc.subjectGenesen_US
dc.subjectResistance and disinfectantsen_US
dc.titleIdentification of genes associated with resistance to disinfectants in the most common resistant bacteria strain of Bacteria isolated from Salon Tools in Ishaka Town, Bushenyi District, Ugandaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Identification of genes associated with resistance to disinfectants in the.pdf
Size:
905.91 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Medical and Health Sciences