Efficient conditions for in vitro establishment and regeneration of disease-free Ugandan farmer-preferred cassava genotypes

Simple item page
dc.contributor.authorApio, Hellen B.
dc.contributor.authorAlicai, Titus
dc.contributor.authorOgwok, Emmanuel
dc.date.accessioned2022-02-15T19:52:55Z
dc.date.available2022-02-15T19:52:55Z
dc.date.issued2021
dc.description.abstractCassava (Manihot esculenta Crantz) is majorly devastated by two viral diseases, cassava brown streak disease (CBSD) and cassava mosaic disease (CMD), resulting in 100% yield loss. Being a clonal plant, nodal cuttings (NC) and shoot apical meristems (SAMs) are the best explants for production of disease free planting materials. In this study, NCs and SAMs were used to determine reliable indicators for successful in vitro establishment of cassava. Eight cassava genotypes were used for the study. Leaf samples were collected from 30 stakes of each of the eight genotypes planted in the screen house. The leaf samples were pooled and screened for presence and/or absence of CBSD and CMD by PCR using virus specific primers. Nodal cuttings were excised from screen house grown plants, surface sterilized to rid-off contaminants and established on Murashige and Skoog (MS) Medium. Using the sprouted stakes, 5-mm sized SAMs were excised, surface sterilized and reduced to 0.5-1 and 2-3 mm sizes. The SAMs were established on MS medium with varying concentrations of plant growth regulators (0.5, 1, 2) ml/L Benzylaminopurine (BAP) and (2, 4) ml/L Naphthalene acetic acid (NAA), Kinetin (K) and BAP respectively. PCR results revealed the pooled leaf samples were free of both CBSD and CMD for all genotypes. Establishment and regeneration of NCs was possible with MS medium for all genotypes. For the SAMs, the concentrations of (2, 4) ml/LBAP followed by 2 ml/LNAA facilitated their establishment and regeneration in comparison to KIN.SAMs of 2-3 mm sizes regenerated better than 0.5 - 1 mm size. Both NCs and SAMs of the different genotypes produced leaves, nodes, roots and there was an increase in plant length. These parameters are critical indicators for in vitro establishment and regeneration of cassava.en_US
dc.identifier.citationApio, H. B., Alicai, T., & Ogwok, E. (2021). Efficient conditions for in vitro establishment and regeneration of disease-free Ugandan farmer-preferred cassava genotypes. DOI: 10.5897/AJB2021.17361en_US
dc.identifier.issn1684-5315
dc.identifier.other10.5897/AJB2021.17361
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/2160
dc.language.isoenen_US
dc.publisherAfrican Journal of Biotechnologyen_US
dc.subjectCassava genotypesen_US
dc.subjectCassava diseasesen_US
dc.subjectShoot apical meristemsen_US
dc.subjectNodal cuttingsen_US
dc.subjectGrowth regulatorsen_US
dc.titleEfficient conditions for in vitro establishment and regeneration of disease-free Ugandan farmer-preferred cassava genotypesen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Efficient conditions for in vitro establishment and.pdf
Size:
853.15 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Agricultural and Veterinary Sciences