Molecular investigation of multiple strain infections in patients with tuberculosis in Mubende district, Uganda

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dc.contributor.authorMuwonge, Adrian
dc.contributor.authorKankya, Clovice
dc.contributor.authorOlea-Popelka, Francisco
dc.contributor.authorBiffa, Demelash
dc.contributor.authorSsengooba, Willy
dc.contributor.authorBerit, Djønne
dc.contributor.authorSkjerve, Eystein
dc.contributor.authorJohansen, Tone B.
dc.date.accessioned2022-02-04T16:19:08Z
dc.date.available2022-02-04T16:19:08Z
dc.date.issued2013
dc.description.abstractMultiple strain tuberculosis (TB) infections are now an acceptable facet of tuberculosis epidemiology. Identification of patients infected with more than one strain gives an insight in disease dynamics at individual and population level. This study therefore aimed at identifying multiple strain infections among TB infected patients. Furthermore, to determine factors associated with multiple strain infections in Mubende district of Uganda. A total of 72 Mycobacterium tuberculosis isolates from patients at Mubende regional referral hospital were characterized using 15 loci MIRU-VNTR, Spoligotyping and deletion analysis. Genotypic and epidemiological data were analyzed using MIRU-VNTR plus, Bionumerics software version 6.1 and an exact logistic regression model respectively. Eight (11.1%) of the 72 patients had mixed TB infections. Five were exclusively pulmonary mixed infections while three had both pulmonary and extra-pulmonary infections (Compartmentalized TB infections). Unlike previous studies that have linked this phenomenon to Beijing strains, multiple strains in this study belonged to T2-Uganda, X2 and T1 lineages. Two of the pulmonary mixed infections were resistant to rifampicin or isoniazid. All except one were HIV positive, newly diagnosed cases and urban residents of Mubende district. The study revealed that one in nine urban dwelling, HIV/TB co-infected patient were infected with more than one M. tuberculosis strains. The molecular findings give indications of a vital component of the disease dynamics that is most likely under looked at clinical level.en_US
dc.identifier.citationMuwonge, A., Kankya, C., Olea-Popelka, F., Biffa, D., Ssengooba, W., Berit, D., ... & Johansen, T. B. (2013). Molecular investigation of multiple strain infections in patients with tuberculosis in Mubende district, Uganda. Infection, Genetics and Evolution, 17, 16-22. http://dx.doi.org/10.1016/j.meegid.2013.03.039en_US
dc.identifier.urihttp://dx.doi.org/10.1016/j.meegid.2013.03.039
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/1918
dc.language.isoenen_US
dc.publisherInfection, Genetics and Evolutionen_US
dc.subjectMixed infectionsen_US
dc.subjectMycobacterium tuberculosisen_US
dc.subjectMubendeen_US
dc.subjectUgandaen_US
dc.titleMolecular investigation of multiple strain infections in patients with tuberculosis in Mubende district, Ugandaen_US
dc.typeArticleen_US
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