Recombination fraction and genetic linkage among key disease resistance genes (Co-42/Phg-2 and Co-5/“P.ult”) in common bean

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dc.contributor.authorOkii, Dennis
dc.contributor.authorBadji, Arfang
dc.contributor.authorOdong, Thomas
dc.contributor.authorTalwana, Herbert
dc.contributor.authorTukamuhabwa, Phinehas
dc.contributor.authorMale, Allan
dc.contributor.authorMukankusi, Clare
dc.contributor.authorGepts, Paul
dc.date.accessioned2022-02-02T10:08:59Z
dc.date.available2022-02-02T10:08:59Z
dc.date.issued2019
dc.description.abstractAnthracnose (Colletotrichum lindemuthianum), Angular leaf spot (Pseudocercospora griseola) and Pythium root rot are important pathogens affecting common bean production in the tropics. A promising strategy to manage these diseases consists of combining several resistance (R) genes into one cultivar. The aim of the study was to determine genetic linkage between gene pairs, Co-42/Phg-2, on bean-chromosome Pv08 and Co-5/“P.ult” on-chromosome Pv07, to increase the efficiency of dual selection of resistance genes for major bean diseases, with molecular markers. The level of recombination was determined by tracking molecular markers for both BC3F6 and F2 generations. Recombination fraction r, among gene pairs, the likelihood of linkage, L(r), and logarithm of odds (LOD) scores were computed using the statistical relationship of likelihood which assumes a binomial distribution. The SCAR marker pair SAB3/PYAA19 for the gene pair Co-5/“P.ult” exhibited moderate linkage (r = 32 cM with a high LOD score of 9.2) for BC3F6 population, but relatively stronger linkage for the F2 population (r = 21 cM with a high LOD score of 18.7). However, the linkage among SCAR marker pair SH18/SN02, for the gene pair Co-42/Phg-2 was incomplete for BC3F6 population (r = 47 cM with a low LOD score of 0.16) as well as F2 population (r = 44 cM with a low LOD score of 0.7). Generally, the weak or incomplete genetic linkage between marker pairs studied showed that all the four genes mentioned earlier have to be tagged with a corresponding linked marker during selection. The approaches used in this study will contribute to two loci linkage mapping techniques in segregating plant populations.en_US
dc.identifier.citationOkii, D., Badji, A., Odong, T., Talwana, H., Tukamuhabwa, P., Male, A., ... & Gepts, P. (2019). Recombination fraction and genetic linkage among key disease resistance genes (Co-42/Phg-2 and Co-5/“P. ult”) in common bean. African journal of biotechnology, 18(29). DOI: 10.5897/AJB2019.16776en_US
dc.identifier.other10.5897/AJB2019.16776
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/1744
dc.language.isoenen_US
dc.publisherAfrican journal of biotechnologyen_US
dc.subjectGenetic linkageen_US
dc.subjectRecombinationen_US
dc.subjectLikelihooden_US
dc.subjectLogarithm of odds (LOD) scoreen_US
dc.subjectSequence characterized amplified regions (SCAR) markersen_US
dc.subjectGenesen_US
dc.titleRecombination fraction and genetic linkage among key disease resistance genes (Co-42/Phg-2 and Co-5/“P.ult”) in common beanen_US
dc.typeArticleen_US
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