Validation of KASP Markers Associated with Hydrogen Cyanide in Fresh Cassava Roots in Uganda Cassava Germplasm

Simple item page
dc.contributor.authorKanaabi, Michael;
dc.contributor.authorMukasa, Settumba B.;
dc.contributor.authorNuwamanya, Ephraim ;
dc.contributor.authorIragaba, Paula;
dc.contributor.authorBaguma, Julius Karubanga;
dc.contributor.authorNanyonjo, Ann Ritah;
dc.contributor.authorWagaba, Henry;
dc.contributor.authorMuhumuza, Nicholas;
dc.contributor.authorNamakula, Fatumah Babirye;
dc.contributor.authorWembabazi, Enoch;
dc.contributor.authorOzimati, Alfred;
dc.contributor.authorKayondo, Ismail Siraj;
dc.contributor.authorEsuma, Williams;
dc.contributor.authorKawuki, Robert S.
dc.date.accessioned2025-04-07T12:24:56Z
dc.date.available2025-04-07T12:24:56Z
dc.date.issued2024-12
dc.description.abstractLow hydrogen cyanide (HCN) concentration is a high-priority trait for cassava varieties targeting their fresh and dry product profiles. To be acceptable, varieties bred and developed for these market segments must meet international safety standards for maximum acceptable residual levels of cyanide in cassava food and food products. The discovery of molecular markers that co-segregate with low HCN has not yet resulted in widespread usage in marker-assisted selection (MAS) in breeding programs. To deploy these HCN markers in regular MAS, assessing their reliability in various genetic backgrounds is crucial. The aim of this study was to assess the predictive accuracy of trait specific markers for HCN. The study used six HCN kompetitive allele-specific polymerase chain reaction (KASP) markers that had previously been developed in a Brazilian population and verified in segregating West African cassava populations. For most markers used in the study, the average call rate was more than 91.7%. Three markers—snpME00404, snpME00405 and snpME00406—showed a significant co-segregation of genotypes with the HCN phenotype. On average, genotypes that carried at least one copy of the favourable allele had lower HCN scores. The proportion of phenotypic variance accounted for by the three most important markers was 14% (snpME00406), 17% (snpME00405) and 27% (snpME00404). The validation of identified HCN SNP-markers marks a significant step in their deployment to support selection and advancement decisions in cassava breeding programs. Agricultural Science Database
dc.description.sponsorshipThis work was supported by the Nextgen cassava breeding project (https://www.nextgencassava.org/ accessed on 1 October 2024) through a grant to Cornell University by Bill & Melinda Gates Foundation (BMGF) (Grant INV-007637) and the UK’s Foreign Commonwealth and Development Office (FCDO). Furthermore, BMGF supported this work through the project on Breeding RTB products for End user Preferences (RTBfoods) (https://rtbfoods.cirad.fr/, accessed on 1 October 2024); Grant INV-008567, formerly OPP1178942 to the French Agricultural Research Center for International Development (CIRAD).
dc.identifier.citationKanaabi, Michael, Settumba B. Mukasa, Ephraim Nuwamanya, et al. 'Validation of KASP Markers Associated with Hydrogen Cyanide in Fresh Cassava Roots in Uganda Cassava Germplasm', Agronomy (Basel), vol. 14/no. 12, (2024), pp. 2765.
dc.identifier.issnISSN 2073-4395
dc.identifier.issnEISSN 2073-4395
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/10392
dc.language.isoen
dc.publisherMDPI AG
dc.titleValidation of KASP Markers Associated with Hydrogen Cyanide in Fresh Cassava Roots in Uganda Cassava Germplasm
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
agronomy-14-02765.pdf
Size:
1.43 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Agricultural and Veterinary Sciences