Development and Validation of a Culture-Based Method Suitable for Monitoring Environmental Survival of Escherichia Coli O157:H7 and Salmonella Enterica Serovar Typhimurium in Developing Countries

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dc.contributor.authorOngeng, Duncan
dc.contributor.authorMuyanja, Charles
dc.contributor.authorRyckeboer, Jaak
dc.contributor.authorGeeraerd, Annemie Helena
dc.contributor.authorSpringael, Dirk
dc.date.accessioned2022-09-07T15:22:25Z
dc.date.available2022-09-07T15:22:25Z
dc.date.issued2011
dc.description.abstractA non-GMO culture-based method suitable for studying the fate of Escherichia coli O157:H7 and Salmonella enterica serovar Typhimurium in an agricultural environment in developing countries was developed and validated. This method is based on the use of spontaneous rifampicin-resistant mutants of non-toxigenic variants of E. coli O157:H7 and S. Typhimurium combined with counting of colony-forming units (CFU) on selective media, i.e., Cefixime–Tellurite–Sorbitol MacConkey agar containing 100 μg/ml rifampicin, 50 μg/ml cycloheximide and 50 μg/ml nystatin for E. coli O157:H7 and Xylose–Lysine–Tergitol-4 agar containing the same antimicrobials for S. Typhimurium. Validation experiments using gfp- and ds-red-labelled derivatives of the rifampicin-resistant mutants in a non-sterile manure-soil matrix demonstrated that the new culture method was effective in the selection and recovery of the test strains without any detectable interference from background contaminants both in the short and long term. The rifampicin-resistant-based culture method designed provides a feasible low-cost option to study environmental survival of E. coli O157:H7 and Salmonella spp. in developing countries.en_US
dc.identifier.citationOngeng, D., Muyanja, C., Ryckeboer, J., Helena Geeraerd, A., & Springael, D. (2011). Development and validation of a culture-based method suitable for monitoring environmental survival of Escherichia coli O157: H7 and Salmonella enterica serovar Typhimurium in developing countries. Annals of microbiology, 61(4), 809-817. https://doi.org/10.1007/s13213-011-0199-4en_US
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4615
dc.language.isoenen_US
dc.publisherAnnals of microbiologyen_US
dc.subjectCulture-based method . Rifampicin-resistant . Eschericha coli O157:H7 . Salmonella Typhimurium . Environmental survival . Developing countriesen_US
dc.titleDevelopment and Validation of a Culture-Based Method Suitable for Monitoring Environmental Survival of Escherichia Coli O157:H7 and Salmonella Enterica Serovar Typhimurium in Developing Countriesen_US
dc.typeArticleen_US
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