An Anti-proteome Nanobody Library Approach Yields a Specific Immunoassay for Trypanosoma congolense Diagnosis Targeting Glycosomal Aldolase

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dc.contributor.authorOdongo, Steven
dc.contributor.authorSterckx, Yann G. J.
dc.contributor.authorStijlemans, BenoƮt
dc.contributor.authorPillay, Davita
dc.contributor.authorMuyldermans, Serge
dc.contributor.authorMagez, Stefan
dc.date.accessioned2023-06-14T14:59:37Z
dc.date.available2023-06-14T14:59:37Z
dc.date.issued2016
dc.description.abstractInfectious diseases pose a severe worldwide threat to human and livestock health. While early diagnosis could enable prompt preventive interventions, the majority of diseases are found in rural settings where basic laboratory facilities are scarce. Under such field conditions, point-of-care immunoassays provide an appropriate solution for rapid and reliable diagnosis. The limiting steps in the development of the assay are the identification of a suitable target antigen and the selection of appropriate high affinity capture and detection antibodies. To meet these challenges, we describe the development of a Nanobody (Nb)-based antigen detection assay generated from a Nb library directed against the soluble proteome of an infectious agent. In this study, Trypanosoma congolense was chosen as a model system.en_US
dc.identifier.citationOdongo, S., Sterckx, Y. G., Stijlemans, B., Pillay, D., Baltz, T., Muyldermans, S., & Magez, S. (2016). An anti-proteome nanobody library approach yields a specific immunoassay for Trypanosoma congolense diagnosis targeting glycosomal aldolase. PLoS neglected tropical diseases, 10(2), e0004420.https://doi.org/10.1371/journal.pntd.0004420en_US
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8920
dc.language.isoenen_US
dc.publisherPLoS neglected tropical diseasesen_US
dc.subjectGlycosomal Aldolaseen_US
dc.subjectTrypanosoma congolenseen_US
dc.subjectAnti-proteomeen_US
dc.subjectNanobodyen_US
dc.titleAn Anti-proteome Nanobody Library Approach Yields a Specific Immunoassay for Trypanosoma congolense Diagnosis Targeting Glycosomal Aldolaseen_US
dc.typeArticleen_US
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