Evaluation of Fingerstick Cryptococcal Antigen Lateral Flow Assay in HIV-Infected Persons: A Diagnostic Accuracy Study

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dc.contributor.authorWilliams, Darlisha A.
dc.contributor.authorKiiza, Tadeo
dc.contributor.authorKwizera, Richard
dc.contributor.authorKiggundu, Reuben
dc.contributor.authorVelamakanni, Sruti
dc.contributor.authorMeya, David B.
dc.contributor.authorRhein, Joshua
dc.contributor.authorBoulware, David R.
dc.date.accessioned2022-08-18T18:33:44Z
dc.date.available2022-08-18T18:33:44Z
dc.date.issued2015
dc.description.abstractCryptococcus neoformans is the most common cause of adult meningitis in sub-Saharan Africa. The cryptococcal antigen (CRAG) lateral flow assay (LFA) has simplified diagnosis as a point-of-care test approved for serum or cerebrospinal fluid (CSF). We evaluated the accuracy of the CRAG LFA using fingerstick whole blood compared with serum/plasma and CSF for diagnosing meningitis.From August 2013 to August 2014, CRAG LFA (IMMY, Norman, Oklahoma) tests were performed on fingerstick whole blood, plasma/serum, and CSF in 207 HIV-infected adults with suspected meningitis in Kampala, Uganda. Venous blood was also collected and centrifuged to obtain serum and/or plasma. CSF was tested after lumbar puncture.Of 207 participants, 149 (72%) had fingerstick CRAG-positive results. There was 100% agreement between fingerstick whole blood and serum/plasma. Of the 149 fingerstick CRAG-positive participants, 138 (93%) had evidence of cryptococcal meningitis with a positive CSF CRAG. Eleven participants (5%) had isolated cryptococcal antigenemia with a negative CSF CRAG and culture, of whom 8 had CSF abnormalities (n = 3 lymphocytic pleocytosis, n = 5 elevated protein, n = 4 increased opening pressure). No persons with cryptococcal meningitis had negative fingersticks.The 100% agreement between whole blood, serum, and plasma CRAG LFA results demonstrates that fingerstick CRAG is a reliable bedside diagnostic test. Using point-of-care CRAG testing simplifies screening large numbers of patients and enables physicians to prioritize on whom to measure CSF opening pressure using manometers.en_US
dc.identifier.citationWilliams, D. A., Kiiza, T., Kwizera, R., Kiggundu, R., Velamakanni, S., Meya, D. B., ... & Boulware, D. R. (2015). Evaluation of fingerstick cryptococcal antigen lateral flow assay in HIV-infected persons: a diagnostic accuracy study. Clinical Infectious Diseases, 61(3), 464-467.https://doi.org/10.1093/cid/civ263en_US
dc.identifier.issn2772-7076
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/4295
dc.language.isoenen_US
dc.publisherClinical Infectious Diseasesen_US
dc.subjectcryptococcal meningitis, cryptococcus, lateral flow assay, HIV, point-of-care systemsen_US
dc.titleEvaluation of Fingerstick Cryptococcal Antigen Lateral Flow Assay in HIV-Infected Persons: A Diagnostic Accuracy Studyen_US
dc.typeArticleen_US
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