Golden bananas in the field: elevated fruit pro-vitamin A from the expression of a single banana transgene

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dc.contributor.authorJean-Yves, Paul
dc.contributor.authorHarjeet, Khanna
dc.contributor.authorKleidon, Jennifer
dc.contributor.authorHoang, Phuong
dc.contributor.authorGeijskes, Jason
dc.contributor.authorDaniells, Jeff
dc.contributor.authorZaplin, Ella
dc.contributor.authorRosenberg, Yvonne
dc.contributor.authorJames, Anthony
dc.contributor.authorBulukani, Mlalazi
dc.contributor.authorPradeep, Deo
dc.contributor.authorArinaitwe, Geofrey
dc.contributor.authorNamanya, Priver
dc.contributor.authorBecker, Douglas
dc.contributor.authorTindamanyire, James
dc.contributor.authorTushemereirwe, Wilberforce
dc.contributor.authorHarding, Robert
dc.contributor.authorDale, James
dc.date.accessioned2021-12-17T15:15:04Z
dc.date.available2021-12-17T15:15:04Z
dc.date.issued2017
dc.description.abstractVitamin A deficiency remains one of the world's major public health problems despite food fortification and supplements strategies. Biofortification of staple crops with enhanced levels of pro-vitamin A (PVA) offers a sustainable alternative strategy to both food fortification and supplementation. As a proof of concept, PVA-biofortified transgenic Cavendish bananas were generated and field trialed in Australia with the aim of achieving a target level of 20 μg/g of dry weight (dw) β-carotene equivalent (β-CE) in the fruit. Expression of a Fe'i banana-derived phytoene synthase 2a (MtPsy2a) gene resulted in the generation of lines with PVA levels exceeding the target level with one line reaching 55 μg/g dw β-CE. Expression of the maize phytoene synthase 1 (ZmPsy1) gene, used to develop ‘Golden Rice 2’, also resulted in increased fruit PVA levels although many lines displayed undesirable phenotypes. Constitutive expression of either transgene with the maize polyubiquitin promoter increased PVA accumulation from the earliest stage of fruit development. In contrast, PVA accumulation was restricted to the late stages of fruit development when either the banana 1-aminocyclopropane-1-carboxylate oxidase or the expansin 1 promoters were used to drive the same transgenes. Wild-type plants with the longest fruit development time had also the highest fruit PVA concentrations. The results from this study suggest that early activation of the rate-limiting enzyme in the carotenoid biosynthetic pathway and extended fruit maturation time are essential factors to achieve optimal PVA concentrations in banana fruit.en_US
dc.identifier.citationPaul, J. Y., Khanna, H., Kleidon, J., Hoang, P., Geijskes, J., Daniells, J., ... & Dale, J. (2017). Golden bananas in the field: elevated fruit pro‐vitamin A from the expression of a single banana transgene. Plant Biotechnology Journal, 15(4), 520-532. https://doi.org/10.1111/pbi.12650en_US
dc.identifier.urihttps://nru.uncst.go.ug/xmlui/handle/123456789/753
dc.language.isoenen_US
dc.publisherPlant Biotechnology Journalen_US
dc.subjectVitamin A deficiency, Uganda, pro-vitamin A, staple food crop, banana, biofortification, genetic modification.en_US
dc.titleGolden bananas in the field: elevated fruit pro-vitamin A from the expression of a single banana transgeneen_US
dc.typeArticleen_US
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