Unmapped exome reads implicate a role for Anelloviridae in childhood HIV-1 long-term non-progression

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dc.contributor.authorMwesigwa, Savannah
dc.contributor.authorWilliams, Lesedi
dc.contributor.authorRetshabile, Gaone
dc.contributor.authorKatagirya, Eric
dc.contributor.authorMboowa, Gerald
dc.contributor.authorMlotshwa, Busisiwe
dc.contributor.authorKyobe, Samuel
dc.contributor.authorKateete, David P.
dc.contributor.authorMujjwiga Wampande, Eddie
dc.contributor.authorWayengera, Misaki
dc.contributor.authorWata Mpoloka, Sununguko
dc.contributor.authorMirembe, Angella N.
dc.contributor.authorKasvosve, Ishmael
dc.contributor.authorMorapedi, Koketso
dc.contributor.authorKisitu, Grace P.
dc.contributor.authorKekitiinwa, Adeodata R.
dc.contributor.authorAnabwani, Gabriel
dc.contributor.authorJoloba, Moses L.
dc.contributor.authorMatovu, Enock
dc.contributor.authorMulindwa, Julius
dc.contributor.authorNoyes, Harry
dc.contributor.authorBotha, Gerrit
dc.contributor.authorBrown, Chester W.
dc.contributor.authorMardon, Graeme
dc.contributor.authorMatshaba, Mogomotsi
dc.contributor.authorHanchard, Neil A.
dc.date.accessioned2023-02-06T20:44:45Z
dc.date.available2023-02-06T20:44:45Z
dc.date.issued2021
dc.description.abstractHuman immunodeficiency virus (HIV) infection remains a significant public health burden globally. The role of viral co-infection in the rate of progression of HIV infection has been suggested but not empirically tested, particularly among children. We extracted and classified 42 viral species from whole-exome sequencing (WES) data of 813 HIV-infected children in Botswana and Uganda categorised as either long-term non-progressors (LTNPs) or rapid progressors (RPs). The Ugandan participants had a higher viral community diversity index compared to Batswana (p = 4.6 × 10−13), and viral sequences were more frequently detected among LTNPs than RPs (24% vs 16%; p = 0.008; OR, 1.9; 95% CI, 1.6–2.3), with Anelloviridae showing strong association with LTNP status (p = 3 × 10−4; q = 0.004, OR, 3.99; 95% CI, 1.74–10.25). This trend was still evident when stratified by country, sex, and sequencing platform, and after a logistic regression analysis adjusting for age, sex, country, and the sequencing platform (p = 0.02; q = 0.03; OR, 7.3; 95% CI, 1.6–40.5). Torque teno virus (TTV), which made up 95% of the Anelloviridae reads, has been associated with reduced immune activation. We identify an association between viral co-infection and prolonged AIDs-free survival status that may have utility as a biomarker of LTNP and could provide mechanistic insights to HIV progression in children, demonstrating the added value of interrogating off-target WES reads in cohort studies.en_US
dc.identifier.citationMwesigwa, S., Williams, L., Retshabile, G., Katagirya, E., Mboowa, G., Mlotshwa, B., ... & Hanchard, N. A. (2021). Unmapped exome reads implicate a role for Anelloviridae in childhood HIV-1 long-term non-progression. NPJ Genomic Medicine, 6(1), 24. https://doi.org/10.1038/s41525-021-00185-wen_US
dc.identifier.urihttps://doi.org/10.1038/s41525-021-00185-w
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7623
dc.language.isoenen_US
dc.publisherNPJ Genomic Medicineen_US
dc.subjectUnmapped exomeen_US
dc.subjectAnelloviridaeen_US
dc.subjectChildhooden_US
dc.subjectHIV-1 long-term non-progressionen_US
dc.titleUnmapped exome reads implicate a role for Anelloviridae in childhood HIV-1 long-term non-progressionen_US
dc.typeArticleen_US
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