Phylogenetic analysis of multidrug resistant E. coli isolates from the urinary tract in Bushenyi district, Uganda using the new Clermont phylotyping method

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dc.contributor.authorOdoki, Martin
dc.contributor.authorAliero, Adamu A.
dc.contributor.authorTibyangye, Julius
dc.contributor.authorOnkoba, Sarah K.
dc.contributor.authorBashir, Alkali
dc.contributor.authorManiga, Josephat N.
dc.contributor.authorEilu, Emmanuel
dc.contributor.authorWampande, Eddie
dc.contributor.authorKato, Charles D.
dc.contributor.authorAgwu, Ezera
dc.contributor.authorBazira, Joel
dc.date.accessioned2022-12-20T23:13:08Z
dc.date.available2022-12-20T23:13:08Z
dc.date.issued2020
dc.description.abstractDue to the increasing rates of multidrug resistance (MDR) among the Enterobacteriaceae that cause urinary tract infections (UTIs), selection of antimicrobial agents for empirical therapy is becoming a major challenge. This study determined the antimicrobial resistance profiles, multidrug resistance profiles, multiple antibiotic resistance indices (MARI), factors associated with MDR UTIs and the phylogenetic groups of MDR Escherichia coli strains isolated from the urinary tract among patients attending hospitals in Bushenyi District, Uganda. In this cross-sectional study, a total of 86 bacterial uropathogens isolated from 267 study participants suspected to have UTIs were subjected to antimicrobial susceptibility tests using the Kirby Bauer Disk diffusion method. Data for the factors associated with MDR were obtained by the use of questionnaires. Phylogenetic groups of the MDR E. coli were determined using the new Clermont method for phylotyping E. coli. Descriptive and multiple logistic regression statistical tools were used to determine phylogenetic groups, and assess for statistically significant relationship between MDR UTIs and factors suspected to be associated with MDR UTIs respectively. The isolates assigned as group B2 9/12 (75.0%), B1 2/5 (40.0%) and A 2/7 (28.6%) by using the old Clermont method could not be phylotyped using the new Clermont method and were grouped as non-typeable strains of E. coli. Our study demonstrated high prevalence of the non-typeable strains of MDR E. coli, we therefore recommend the use of modern DNA sequencing-based approaches which is the gold standard for genotyping bacteria, that this current study could not afforden_US
dc.identifier.citationMartin, O., Adamu, A. A., Julius, T., Sarah, K. O., Bashir, A., Josephat, N. M., ... & Joel, B. (2020). Phylogenetic analysis of multidrug resistant E. coli isolates from the urinary tract in Bushenyi district, Uganda using the new Clermont phylotyping method. African Journal of Microbiology Research, 14(2), 51-64.en_US
dc.identifier.urihttps://academicjournals.org/journal/AJMR/article-full-text-pdf/A20668A62773
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/6463
dc.language.isoenen_US
dc.publisherAfrican Journal of Microbiology Researchen_US
dc.subjectPhylogenetic analysisen_US
dc.subjectbacterial urinary tract infectionsen_US
dc.subjectfactors associated withen_US
dc.titlePhylogenetic analysis of multidrug resistant E. coli isolates from the urinary tract in Bushenyi district, Uganda using the new Clermont phylotyping methoden_US
dc.typeArticleen_US
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