Genetic diversity of Ascaris in southwestern Uganda

Simple item page
dc.contributor.authorBetson, Martha
dc.contributor.authorNejsum, Peter
dc.contributor.authorLlewellyn-Hughes, Julia
dc.contributor.authorGriffin, Claire
dc.contributor.authorAtuhaire, Aaron
dc.contributor.authorArinaitwe, Moses
dc.contributor.authorAdriko, Moses
dc.contributor.authorRuggiana, Andrew
dc.contributor.authorTuryakira, Grace
dc.contributor.authorKabatereine, Narcis B.
dc.contributor.authorStothard, Russell
dc.date.accessioned2023-01-25T12:18:32Z
dc.date.available2023-01-25T12:18:32Z
dc.date.issued2012
dc.description.abstractDespite the common occurrence of ascariasis in southwestern Uganda, helminth control in the region has been limited. To gain further insights into the genetic diversity of Ascaris in this area, a parasitological survey in mothers (n = 41) and children (n = 74) living in two villages, Habutobere and Musezero, was carried out. Adult Ascaris worms were collected from infected individuals by chemo-expulsion using pyrantel pamoate treatment. Genetic diversity within these worms was assessed by inspection of DNA sequence variation in a mitochondrial marker and length polymorphism at microsatellite loci. Overall prevalence of ascariasis was 42.5% in mothers and 30.4% in their children and a total of 98 worms was examined from 18 hosts. Sequence analysis of a portion of the mitochondrial cytochrome c oxidase subunit 1 gene revealed 19 different haplotypes, 13 of which had not been previously encountered. Microsatellite analysis using eight loci provided evidence for high gene flow between worm populations from the two villages but comparing these worms with others obtained in a prior study on Unguja, Zanzibar, confirmed little genetic exchange and mixing of worm populations between the two areas. By adding to our understanding of the genetic diversity of Ascaris in Africa, this study provides useful information for monitoring changes in parasite population structure in the face of ongoing and future control.en_US
dc.identifier.citationBetson, M., Nejsum, P., Llewellyn-Hughes, J., Griffin, C., Atuhaire, A., Arinaitwe, M., ... & Stothard, J. R. (2012). Genetic diversity of Ascaris in southwestern Uganda. Transactions of the Royal Society of Tropical Medicine and Hygiene, 106(2), 75-83. doi:10.1016/j.trstmh.2011.10.011en_US
dc.identifier.other10.1016/j.trstmh.2011.10.011
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7206
dc.language.isoenen_US
dc.publisherTransactions of the Royal Society of Tropical Medicine and Hygieneen_US
dc.subjectAscarisen_US
dc.subjectEvolutionen_US
dc.subjectCytochrome c oxidase subunit 1en_US
dc.subjectMicrosatelliteen_US
dc.subjectPopulation geneticsen_US
dc.titleGenetic diversity of Ascaris in southwestern Ugandaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Genetic diversity of Ascaris in southwestern Uganda.pdf
Size:
310.96 KB
Format:
Adobe Portable Document Format
Description:
Article
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Agricultural and Veterinary Sciences