Lipid storage by adipose tissue macrophages regulates systemic glucose tolerance

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dc.contributor.authorAouadi, Myriam
dc.contributor.authorVangala, Pranitha
dc.contributor.authorYawe, Joseph C.
dc.contributor.authorTencerova, Michaela
dc.contributor.authorCohen, Jessica L.
dc.contributor.authorCzech, Michael P.
dc.date.accessioned2023-04-13T19:47:53Z
dc.date.available2023-04-13T19:47:53Z
dc.date.issued2014
dc.description.abstractProinflammatory pathways in adipose tissue macrophages (ATMs) can impair glucose tolerance in obesity, but ATMs may also be beneficial as repositories for excess lipid that adipocytes are unable to store. To test this hypothesis, we selectively targeted visceral ATMs in obese mice with siRNA against lipoprotein lipase (LPL), leaving macrophages within other organs unaffected. Selective silencing of ATM LPL decreased foam cell formation in visceral adipose tissue of obese mice, consistent with a reduced supply of fatty acids from VLDL hydrolysis. Unexpectedly, silencing LPL also decreased the expression of genes involved in fatty acid uptake (CD36) and esterification in ATMs. This deficit in fatty acid uptake capacity was associated with increased circulating serum free fatty acids. Importantly, ATM LPL silencing also caused a marked increase in circulating fatty acid-binding protein-4, an adipocyte-derived lipid chaperone previously reported to induce liver insulin resistance and glucose intolerance. Consistent with this concept, obese mice with LPL-depleted ATMs exhibited higher hepatic glucose production from pyruvate and glucose intolerance. Silencing CD36 in ATMs also promoted glucose intolerance. Taken together, the data indicate that LPL secreted by ATMs enhances their ability to sequester excess lipid in obese mice, promoting systemic glucose tolerance.en_US
dc.identifier.citationAouadi, M., Vangala, P., Yawe, J. C., Tencerova, M., Nicoloro, S. M., Cohen, J. L., ... & Czech, M. P. (2014). Lipid storage by adipose tissue macrophages regulates systemic glucose tolerance. American Journal of Physiology-Endocrinology and Metabolism, 307(4), E374-E383.https://doi.org/10.1152/ajpendo.00187.2014en_US
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8467
dc.language.isoenen_US
dc.publisherAmerican Journal of Physiology-Endocrinology and Metabolismen_US
dc.subjectFoam cellsen_US
dc.subjectsiRNAen_US
dc.subjectAdipose tissue macrophagesen_US
dc.subjectInsulin resistanceen_US
dc.titleLipid storage by adipose tissue macrophages regulates systemic glucose toleranceen_US
dc.typeArticleen_US
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