Molecular characterization and phylogenetic study of African swine fever virus isolates from recent outbreaks in Uganda (2010–2013)

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dc.contributor.authorKalenzi Atuhaire, David
dc.contributor.authorAfayoa, Mathias
dc.contributor.authorOchwo, Sylvester
dc.contributor.authorMwesigwa, Savannah
dc.contributor.authorOkuni, Julius Boniface
dc.contributor.authorOlaho-Mukani, William
dc.contributor.authorOjok, Lonzy
dc.date.accessioned2023-02-06T19:52:37Z
dc.date.available2023-02-06T19:52:37Z
dc.date.issued2013
dc.description.abstractAfrican swine fever (ASF) is a highly lethal and economically significant disease of domestic pigs in Eastern Africa particularly in Uganda where outbreaks regularly occur. Sequence analysis of variable genome regions have been extensively used for molecular epidemiological studies of African swine fever virus (ASFV) isolates. By combining p72, P54 and pB602L (CVR), a high level resolution approach is achieved for viral discrimination. The major aim of this study therefore, was to investigate the genetic relatedness of ASF outbreaks that occurred between 2010 and 2013 in Uganda to contribute to the clarification of the epidemiological situation over a four year period. Methods: Tissue samples from infected domestic pigs associated with an ASF outbreak from 15 districts in Uganda were confirmed as being infected with ASFV using a p72 gene-based polymerase chain reaction amplification (PCR) assay recommended by OIE. The analysis was conducted by genotyping based on sequence data from three single copy ASFV genes. The E183L gene encoding the structural protein P54 and part of the gene encoding the p72 protein was used to delineate genotypes. Intra-genotypic resolution of viral relationships was achieved by analysis of tetramer amino acid repeats within the hypervariable CVR of the B602L gene. Results: Twenty one (21) ASF outbreaks were confirmed by the p72 ASF diagnostic PCR, however; only 17 isolates were successfully aligned after sequencing. Our entire isolates cluster with previous ASF viruses in genotype IX isolated in Uganda and Kenya using p72 and P54 genes. Analysis of the CVR gene generated three sub-groups one with 23 tetrameric amino acid repeats (TRS) with an additional CAST sequence, the second with 22 TRS while one isolate Ug13. Kampala1 had 13 TRS. Conclusion: We identified two new CVR subgroups different from previous studies. This study constitutes the first detailed assessment of the molecular epidemiology of ASFV in domestic pigs in the different regions of Uganda.en_US
dc.identifier.citationAtuhaire, D. K., Afayoa, M., Ochwo, S., Mwesigwa, S., Okuni, J. B., Olaho-Mukani, W., & Ojok, L. (2013). Molecular characterization and phylogenetic study of African swine fever virus isolates from recent outbreaks in Uganda (2010–2013). Virology journal, 10(1), 1-8. doi:10.1186/1743-422X-10-247en_US
dc.identifier.other10.1186/1743-422X-10-247
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7617
dc.language.isoenen_US
dc.publisherVirology journalen_US
dc.subjectAfrican swine fever virusen_US
dc.subjectGenotypingen_US
dc.subjectP54en_US
dc.subjectp72en_US
dc.subjectpB602L (central variable region) sequencingen_US
dc.titleMolecular characterization and phylogenetic study of African swine fever virus isolates from recent outbreaks in Uganda (2010–2013)en_US
dc.typeArticleen_US
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