Field Evaluation of LED Fluorescence Microscopy for Demonstration of Trypanosoma brucei rhodesiense in Patient Blood

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dc.contributor.authorMatovu, Enock
dc.contributor.authorEdielu, Andrew
dc.contributor.authorOjom, James
dc.contributor.authorNanteza, Ann
dc.contributor.authorKato, Charles Drago
dc.contributor.authorNdung’u, Joseph Mathu
dc.date.accessioned2023-03-07T09:26:12Z
dc.date.available2023-03-07T09:26:12Z
dc.date.issued2019
dc.description.abstractDiagnosis of Trypanosoma brucei rhodesiense human African trypanosomiasis requires demonstration of parasites in body fluids by microscopy. The microscopy methods that are routinely used are difficult to deploy in resource-limited settings due to practical challenges, including lengthy and tedious procedures, and the need for specific equipment to centrifuge samples in glass capillary tubes. We report here on a study that was conducted in a rural region of eastern Uganda to evaluate new methods that take advantage of a field-deployable LED fluorescence microscope. Examination of acridine orange-stained blood smears by LED fluorescence microscopy resulted in a diagnostic accuracy that was similar to that of routine methods, while the time needed to identify parasites was shortened significantly. These findings make these new microscopy methods attractive alternatives to procedures that are currently used for diagnosis of T. b. rhodesiense human African trypanosomiasis.en_US
dc.identifier.citationMatovu, E., Edielu, A., Ojom, J., Nanteza, A., Kato, C. D., Biéler, S., & Ndung’u, J. M. (2019). Field evaluation of LED fluorescence microscopy for demonstration of Trypanosoma brucei rhodesiense in patient blood. Microscopy Research, 7(1), 1-9.https://doi.org/10.4236/mr.2019.71001en_US
dc.identifier.issn2329-3314
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8105
dc.language.isoenen_US
dc.publisherMicroscopy Researchen_US
dc.subjectHuman African Trypanosomiasisen_US
dc.subjectRhodesienseen_US
dc.subjectLED Fluorescence Microscopyen_US
dc.subjectRed Blood Cell Lysisen_US
dc.subjectDiagnosisen_US
dc.titleField Evaluation of LED Fluorescence Microscopy for Demonstration of Trypanosoma brucei rhodesiense in Patient Blooden_US
dc.typeArticleen_US
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