Concise gene signature for point-of-care classification of tuberculosis

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dc.contributor.authorMaertzdorf, Jeroen
dc.contributor.authorMcEwen, Gayle McEwenGayle
dc.contributor.authorWeine, January
dc.contributor.authorMayanja-Kizza, Harriet
dc.contributor.authorKaufmann, Stefan HE
dc.date.accessioned2022-05-25T09:10:08Z
dc.date.available2022-05-25T09:10:08Z
dc.date.issued2016
dc.description.abstractThere is an urgent need for new tools to combat the ongoing tuberculosis (TB) pandemic. Gene expression profiles based on blood signatures have proved useful in identifying genes that enable classification of TB patients, but have thus far been complex. Using real-time PCR analysis, we evaluated the expression profiles from a large panel of genes in TB patients and healthy individuals in an Indian cohort. Classification models were built and validated for their capacity to discriminate samples from TB patients and controls within this cohort and on external independent gene expression datasets. A combination of only four genes distinguished TB patients from healthy individuals in both cross-validations and on separate validation datasets with very high accuracy. An external validation on two distinct cohorts using a real-time PCR setting confirmed the predictive power of this 4-gene tool reaching sensitivity scores of 88% with a specificity of around 75%. Moreover, this gene signature demonstrated good classification power in HIV+ populations and also between TB and several other pulmonary diseases. Here we present proof of concept that our 4-gene signature and the top classifier genes from our models provide excellent candidates for the development of molecular point-of-care TB diagnosis in endemic areas.en_US
dc.identifier.citationMaertzdorf, J., McEwen, G., Weiner 3rd, J., Tian, S., Lader, E., Schriek, U., ... & Kaufmann, S. H. (2016). Concise gene signature for point‐of‐care classification of tuberculosis. EMBO molecular medicine, 8(2), 86-95.https://doi.org/10.15252/emmm.201505790en_US
dc.identifier.issn1757-4684
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/3302
dc.language.isoenen_US
dc.publisherEMBO molecular medicineen_US
dc.subjectdisease classification; genomics; molecular diagnosis; real-time PCR; tuberculosisen_US
dc.titleConcise gene signature for point-of-care classification of tuberculosisen_US
dc.typeArticleen_US
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