Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing

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dc.contributor.authorVasoya, Deepali
dc.contributor.authorLaw, Andy
dc.contributor.authorMotta, Paolo
dc.contributor.authorYu, Mingyan
dc.contributor.authorMuwonge, Adrian
dc.contributor.authorCook, Elizabeth
dc.contributor.authorLi, Xiaoying
dc.contributor.authorBryson, Karen
dc.contributor.authorMacCallam, Amanda
dc.contributor.authorSitt, Tatjana
dc.contributor.authorToye, Philip
dc.contributor.authorBronsvoort, Barend
dc.contributor.authorWatson, Mick
dc.contributor.authorMorrison, W. Ivan
dc.contributor.authorConnelley, Timothy
dc.date.accessioned2023-02-08T17:27:56Z
dc.date.available2023-02-08T17:27:56Z
dc.date.issued2016
dc.description.abstractThe major histocompatibility complex (MHC) region contains many genes that are key regulators of both innate and adaptive immunity including the polymorphicMHCI and MHCII genes. Consequently, the characterisation of the repertoire of MHC genes is critical to understanding the variation that determines the nature of immune responses. Our current knowledge of the bovine MHCI repertoire is limited with only the Holstein-Friesian breed having been studied in any depth. Traditional methods of MHCI genotyping are of low resolution and laborious and this has been a major impediment to a more comprehensive analysis of the MHCI repertoire of other cattle breeds. Next-generation sequencing (NGS) technologies have been used to enable high throughput and much higher resolution MHCI typing in a number of species. In this study we have developed a MiSeq platform approach and requisite bioinformatics pipeline to facilitate typing of bovineMHCI repertoires. The method was validated initially on a cohort of Holstein-Friesian animals and then demonstrated to enable characterisation of MHCI repertoires in African cattle breeds, for which there was limited or no available data. During the course of these studies we identified >140 novel classical MHCI genes and defined 62 novel MHCI haplotypes, dramatically expanding the known bovine MHCI repertoire.en_US
dc.identifier.citationVasoya, D., Law, A., Motta, P., Yu, M., Muwonge, A., Cook, E., ... & Connelley, T. (2016). Rapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencing. Immunogenetics, 68, 765-781. DOI 10.1007/s00251-016-0945-7en_US
dc.identifier.other10.1007/s00251-016-0945-7
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/7651
dc.language.isoenen_US
dc.publisherImmunogeneticsen_US
dc.subjectCattleen_US
dc.subjectMHCIen_US
dc.subjectNext-generation sequencingen_US
dc.titleRapid identification of bovine MHCI haplotypes in genetically divergent cattle populations using next-generation sequencingen_US
dc.typeArticleen_US
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