Population genetic structure and temporal stability among Trypanosoma brucei rhodesiense isolates in Uganda

Simple item page
dc.contributor.authorKato, Charles D.
dc.contributor.authorAlibu, Vincent P.
dc.contributor.authorNanteza, Ann
dc.contributor.authorMugasa, Claire M.
dc.contributor.authorMatovu, Enock
dc.date.accessioned2023-03-07T15:25:25Z
dc.date.available2023-03-07T15:25:25Z
dc.date.issued2016
dc.description.abstractThe population structure and role of genetic exchange in African trypanosomes have been previously analyzed albeit with contradictory findings. To further investigate the role of genetic polymorphism on the population genetic structure of Trypanosoma b. rhodesiense, we hypothesized that parasite genotypes are clonal and stable over time. We have undertaken a microsatellite marker analysis of T. b. rhodesiense isolates in a relatively new active HAT focus in Uganda (Kaberamaido-Dokolo-Amolatar) over a six-year period (2006–2012). We amplified six microsatellite markers by PCR directly from blood spotted FTA cards following whole genome amplification. The majority of loci demonstrated an excess of heterozygosity (Ho > He, FIS < 0). We identified 26 unique genotypes among the 57 isolates, accounting for 45.6 % genotypic polymorphism. The presence of a high proportion of samples with repeated genotypes (54.4 %, 31/57), disagreement with Hardy-Weinberg equilibrium, and significant linkage disequilibrium between loci pairs, provide evidence that T. b. rhodesiense isolates from this focus are clonal. Our results show low values of FST’ (0–0.115) indicating negligible genetic differentiation across temporal isolates. Furthermore, predominant genotypes isolated in 2006 were still detectable in 2012. Our findings confirm the notion that endemicity is maintained by stable genotypes rather than an influx of new genotypes. Our results have considerable importance in understanding and tracking the spread of sleeping sickness with significant implication to disease control.en_US
dc.identifier.citationKato, C. D., Alibu, V. P., Nanteza, A., Mugasa, C. M., & Matovu, E. (2016). Population genetic structure and temporal stability among Trypanosoma brucei rhodesiense isolates in Uganda. Parasites & Vectors, 9, 1-8.https://doi.org/10.1186/s13071-016-1542-1en_US
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/8118
dc.language.isoenen_US
dc.publisherParasites & Vectorsen_US
dc.subjectPopulationen_US
dc.subjectGenetic structureen_US
dc.subjectTrypanosoma brucei rhodesienseen_US
dc.titlePopulation genetic structure and temporal stability among Trypanosoma brucei rhodesiense isolates in Ugandaen_US
dc.typeArticleen_US
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Population genetic structure and temporal stability among Trypanosoma brucei rhodesiense isolates in Uganda.pdf
Size:
648.65 KB
Format:
Adobe Portable Document Format
Description:
Population genetic structure and temporal stability among Trypanosoma brucei rhodesiense isolates in Uganda
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Agricultural and Veterinary Sciences