Dried blood spot improves global access to aquaporin-4-IgG testing for neuromyelitis optica

Simple item page
dc.contributor.authorVorasoot, Nisa
dc.contributor.authorAbdulrahman, Yahya J
dc.contributor.authorMateen, Farrah
dc.contributor.authorFryer, James P
dc.contributor.authorRedenbaugh, Vyanka
dc.contributor.authorSagen, Jessica A
dc.contributor.authorMusubire, Abdu K
dc.contributor.authorJenkins, Sarah M
dc.contributor.authorGorsh, Amy P
dc.contributor.authorChen, John J
dc.contributor.authorZekeridou, Anastasia
dc.contributor.authorMcKeon, Andrew
dc.contributor.authorFlanagan, Eoin P
dc.contributor.authorMills, John R
dc.contributor.authorPittock, Sean J
dc.date.accessioned2024-10-21T09:36:31Z
dc.date.available2024-10-21T09:36:31Z
dc.date.issued2024-07-30
dc.description.abstractThis study aimed to evaluate the diagnostic accuracy of dried blood spot (DBS) compared with conventional serum Aquaporin-4-IgG (AQP4-IgG) testing.OBJECTIVEThis study aimed to evaluate the diagnostic accuracy of dried blood spot (DBS) compared with conventional serum Aquaporin-4-IgG (AQP4-IgG) testing.Prospective multicenter diagnostic study was conducted between April 2018 and October 2023 across medical centers in the United States, Uganda, and the Republic of Guinea. Neuromyelitis optica spectrum disorder (NMOSD) patients and controls collected blood on filter paper cards along with concurrent serum samples. These samples underwent analysis using flow cytometric live-cell-based assays (CBA) and enzyme-linked immunosorbent assay (ELISA) to determine AQP4 serostatus. The accuracy of AQP4-IgG detection between DBS and serum (gold standard) was compared.METHODSProspective multicenter diagnostic study was conducted between April 2018 and October 2023 across medical centers in the United States, Uganda, and the Republic of Guinea. Neuromyelitis optica spectrum disorder (NMOSD) patients and controls collected blood on filter paper cards along with concurrent serum samples. These samples underwent analysis using flow cytometric live-cell-based assays (CBA) and enzyme-linked immunosorbent assay (ELISA) to determine AQP4 serostatus. The accuracy of AQP4-IgG detection between DBS and serum (gold standard) was compared.Among 150 participants (47 cases, 103 controls), there was a strong correlation between DBS and serum samples (Spearman's correlation coefficient of 0.82). The AUC was 0.97 (95% CI: 0.92-0.99). AQP4-IgG detection through DBS showed 87.0% sensitivity (95% CI: 0.74-0.95) and 100% specificity (95% CI: 0.96-1.00) using CBA, and 65.2% sensitivity (95% CI: 0.43-0.84) and 95.2% specificity (95% CI: 0.76-0.99) using ELISA. Serum ELISA demonstrated 69.6% sensitivity (95% CI: 0.47-0.87) and 98.4% specificity (95% CI: 0.91-0.99). The stability of DBS in detecting AQP4-IgG persisted over 24 months for most cases.RESULTSAmong 150 participants (47 cases, 103 controls), there was a strong correlation between DBS and serum samples (Spearman's correlation coefficient of 0.82). The AUC was 0.97 (95% CI: 0.92-0.99). AQP4-IgG detection through DBS showed 87.0% sensitivity (95% CI: 0.74-0.95) and 100% specificity (95% CI: 0.96-1.00) using CBA, and 65.2% sensitivity (95% CI: 0.43-0.84) and 95.2% specificity (95% CI: 0.76-0.99) using ELISA. Serum ELISA demonstrated 69.6% sensitivity (95% CI: 0.47-0.87) and 98.4% specificity (95% CI: 0.91-0.99). The stability of DBS in detecting AQP4-IgG persisted over 24 months for most cases.The DBS represents a viable alternative for detecting AQP4-IgG in resource-limited settings to diagnose NMOSD, offering high sensitivity and specificity comparable to serum testing. Moreover, DBS has low shipping costs, is easy to administer, and is suitable for point-of-care testing.INTERPRETATIONThe DBS represents a viable alternative for detecting AQP4-IgG in resource-limited settings to diagnose NMOSD, offering high sensitivity and specificity comparable to serum testing. Moreover, DBS has low shipping costs, is easy to administer, and is suitable for point-of-care testing. MEDLINE - Academic
dc.identifier.citationVorasoot, Nisa, Yahya J. Abdulrahman, Farrah Mateen, et al. 'Dried Blood Spot Improves Global Access to aquaporin‐4‐ IgG Testing for Neuromyelitis Optica', Annals of Clinical and Translational Neurology, (2024), .
dc.identifier.issnISSN 2328-9503
dc.identifier.issnEISSN 2328-9503
dc.identifier.urihttps://nru.uncst.go.ug/handle/123456789/9663
dc.language.isoen
dc.publisherWiley Periodicals LLC
dc.titleDried blood spot improves global access to aquaporin-4-IgG testing for neuromyelitis optica
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Thumbnail Image
Name:
Ann Clin Transl Neurol - 2024 - Vorasoot - Dried blood spot improves global access to aquaporin‐4‐IgG testing for.pdf
Size:
1.27 MB
Format:
Adobe Portable Document Format
Download
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.71 KB
Format:
Item-specific license agreed upon to submission
Description:
Download
Collections
Medical and Health Sciences